Abstract
Kluyveromyces marxianus GX-15 was mutated multiple times by alternately treatment with UV irradiation and NTG for two cycles. Four mutant strains with improved ethanol yield were obtained. The maximum ethanol concentration, ethanol yield coefficient and theoretical ethanol yield of the best mutant strain, GX-UN120, was 69 g/l, 0.46 g/g and 91%, respectively, when fermenting 150 g glucose/l at 40°C. The corresponding values for GX-15 were 58 g/l, 0.39 g/g and 76%, respectively. GX-UN120 grew well in 11% (v/v) of ethanol, while GX-15 could not grow when ethanol was greater than 8% (v/v).
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References
Alper H, Moxley J, Nevoigt E, Fink GR, Stephanopoulos G (2006) Engineering yeast transcription machinery for improved ethanol tolerance and production. Science 314:1565–1568
Araque E, Parra C, Rodriguez M, Freer J, Baeza J (2008) Selection of thermotolerant yeast strains Saccharomyces cerevisiae for bioethanol production. Enzyme Microb Technol 43:120–123
Babu P, North SJ, Jang-Lee J, Chalabi S, Mackerness K, Stowell SR, Cummings RD, Rankin S, Dell A, Haslam SM (2009) Structural characterisation of neutrophil glycans by ultra sensitive mass spectrometric glycomics methodology. Glycoconj J 26:975–986
Bai FW, Anderson WA, Moo-Young M (2008) Ethanol fermentation technologies from sugar and starch feedstocks. Biotechnol Adv 26:89–105
Banat IM, Nigam P, Singh D, Marchant R, McHale AP (1998) Review: Ethanol production at elevated temperatures and alcohol concentrations: part I—yeasts in general. World J Microbiol Biotechnol 14:809–821
Cho JY, Jeffries TW (1999) Transcriptional control of ADH genes in the xylose-fermenting yeast Pichia stipitis. Appl Environ Microbiol 65:2363–2368
Fonseca GG, Heinzle E, Wittmann C, Gombert AK (2008) The yeast Kluyveromyces marxianus and its biotechnological potential. Appl Microbiol Biotechnol 79:339–354
Govindaswamy S, Vane LM (2007) Kinetics of growth and ethanol production on different carbon substrates using genetically engineered xylose-fermenting yeast. Bioresour Technol 98:677–685
Hack CJ, Marchant R (1998) Ethanol adaptation in a thermotolerant yeast strain Kluyveromyces marxianus IMB3. J Ind Microbiol Biotechnol 20:227–231
Lertwattanasakul N, Sootsuwan K, Limtong S, Thanonkeo P, Yamada M (2007) Comparison of the gene expression patterns of alcohol dehydrogenase isozymes in the thermotolerant yeast Kluyveromyces marxianus and their physiological functions. Biosci Biotechnol Biochem 71:1170–1182
Limtong S, Sringiew C, Yongmanitchai W (2007) Production of fuel ethanol at high temperature from sugar cane juice by a newly isolated Kluyveromyces marxianus. Bioresour Technol 98:3367–3374
Shevchenko A, Wilm M, Vorm O, Mann M (1996) Mass spectrometric sequencing of proteins from silver-stained polyacrylamide gels. Anal Chem 68:850–858
Sridhar M, Sree NK, Rao LV (2002) Effect of UV radiation on thermotolerance, ethanol tolerance and osmotolerance of Saccharomyces cerevisiae VS1 and VS3 strains. Bioresour Technol 83:199–202
Wilkins MR, Mueller M, Eichling S, Banat IM (2008) Fermentation of xylose by the thermotolerant yeast strains Kluyveromyces marxianus IMB2, IMB4, and IMB5 under anaerobic conditions. Process Biochem 43:346–350
Acknowledgments
This work was financially supported by the National High Technology Research and Development 863 Program of China (Grant No. 2007AA021307) and the Guangxi Scientific Research and Technology Project (Grant No. 0895003-5-1).
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Pang, Zw., Liang, Jj., Qin, Xj. et al. Multiple induced mutagenesis for improvement of ethanol production by Kluyveromyces marxianus . Biotechnol Lett 32, 1847–1851 (2010). https://doi.org/10.1007/s10529-010-0384-8
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DOI: https://doi.org/10.1007/s10529-010-0384-8