Abstract
Burkholderia vietnamiensis P418 is a plant growth-promoting rhizobacteria. A chitinase gene from Bacillus subtilis was cloned and stably integrated into the chromosome of using the transposon delivery vector, pUTkm1. Chitinase activity was detected in recombinant P418-37 but not in wild type P418. Recombinant P418-37 retained the in vitro growth rate, N2-fixation and phosphate and potassium-solubilizing characteristics of the wild type. P418-37 significantly (P < 0.05) increased in vitro inhibition of the plant pathogenic fungi Rhizoctonia solani, Fusarium oxysporum f.sp. vasinfectum, Rhizoctonia cerealis, Bipolaris sorokiniana, Verticillium dahliae and Gaeumannomyces graminis var. tritici compared with P418. In planta disease suppression assays indicated that P418-37 significantly (P < 0.05) enhanced suppression of wheat sheath blight (R. cerealis), cotton Fusarium wilt (F. oxysporium f.sp. vasinfectum) and tomato gray mould (Botrytis cinerea), relative to the wild type.
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Acknowledgments
This work was supported by the grants from the Ministry of Science and Technology of China (2010DFA32330 and 2011AA10A210). We thank Dr. Dmitry Bazhanov (Institute of Genetics and Cytology, National Academy of Sciences of Belarus) for Burk. vietnamiensis P418 and Dr. Liqun Zhang (China Agricultural University) for plasmid pUTkm1.
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Zhang, X., Huang, Y., Harvey, P.R. et al. Enhancing plant disease suppression by Burkholderia vietnamiensis through chromosomal integration of Bacillus subtilis chitinase gene chi113 . Biotechnol Lett 34, 287–293 (2012). https://doi.org/10.1007/s10529-011-0760-z
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DOI: https://doi.org/10.1007/s10529-011-0760-z