Abstract
Pancreatic cancer remains the common cancer with the worst prognosis because of its late diagnosis and extensive metastasis. This study aimed to investigate the effects of GABRP on pancreatic cancer metastasis and the molecular mechanism. The expression of GABRP was measured using the quantitative real-time PCR and western blot. The biological behaviors of cancer cells were assessed using the cell counting kit-8, Transwell assay, and western blot. The regulation of GABRP on the MEK/ERK pathway was detected by western blot. The results indicated that GABRP was overexpressed in pancreatic cancer tissues and cells. Knockdown of GABRP suppressed cell viability, invasion, migration, and epithelial–mesenchymal transition (EMT), whereas GABRP overexpression facilitated these biological behaviors. Inactivation of the MEK/ERK pathway reversed the effects on cellular processes induced by GABRP. Moreover, silencing of GABRP inhibited tumor growth. In conclusion, GABRP promoted the progression of pancreatic cancer by facilitating cell metastasis and tumor growth via activating the MEK/ERK pathway. The findings suggest that GABRP has the potential to be a therapeutic target for the metastatic pancreatic cancer.
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Data are available upon reasonable request from corresponding author.
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All authors contributed to the study conception and design. Material preparation, data collection, and analysis were performed by YM, SG, WC, WJ, ZL, JH, and ZX. The first draft of the manuscript was written by YM and all authors commented on previous versions of the manuscript. All authors read and approved the final manuscript.
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Meng, Y., Li, R., Geng, S. et al. GABRP Promotes the Metastasis of Pancreatic Cancer by Activation of the MEK/ERK Signaling Pathway. Biochem Genet 62, 242–253 (2024). https://doi.org/10.1007/s10528-023-10410-z
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DOI: https://doi.org/10.1007/s10528-023-10410-z