Abstract
SnRK2 protein kinase family plays an important role in plant response to abiotic stress and has been identified in various plants. This study aimed to identify SnRK2 genes in tobacco and systematically analyze their expression under abscisic acid treatment and abiotic stress. We identified 22 NtSnRK2 members, which were divided into three groups and located on 13 chromosomes, mainly at both ends of the chromosomes; additionally, 11 duplicated NtSnRK2 gene pairs were observed. Phylogenetic analysis showed that these SnRK2 members were divided into three groups in tobacco. The motifs of NtSnRK2 proteins in the same group were highly similar. Subcellular localization indicated that NtSnRK2s in Group3 were present in the nucleus, cytomembrane, and cytoplasm. Gene expression pattern analysis revealed that NtSnRK2 genes played a role in the responses to several abiotic stresses (salt, drought, and low-temperature stress), indicating that they are widely involved in the adaptation of tobacco to adverse environmental conditions.
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Acknowledgements
We thank Nanping Branch of Fujian Tobacco Company, Chongqing Branch of China National Tobacco Corporation, and Henan Agricultural University for the financial support and technical assistance. We also appreciate the reviewers and editors for their patience with regard to this work.
Funding
This work was supported by Chongqing Branch of China National Tobacco Corporation (A2020NY01-1303-1) and Fujian Branch of China National Tobacco Corporation (2021350000240019).
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FH and JHL conceived and designed the experiments. JYS, JTM, JWL, and CJL performed the experiments and participated in data analysis. XWZ and JCL performed the qRT-PCR experiments. YC revised the manuscript.
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Li, J., Song, J., Li, C. et al. Genome-Wide Identification and Expression Profile Analysis of the SnRK2 Gene Family in Nicotiana tabacum. Biochem Genet 60, 1511–1526 (2022). https://doi.org/10.1007/s10528-021-10170-8
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DOI: https://doi.org/10.1007/s10528-021-10170-8