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Description of Komagataella mondaviorum sp. nov., a new sibling species of Komagataella (Pichia) pastoris

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Abstract

Five methylotrophic strains (UCDFST 71-1024T, UCDFST 54-11.16, UCDFST 54-11.141, UCDFST 68-967.1 and UCDFST 74-1030) from the Phaff Yeast Culture Collection (University of California Davis, USA) that were originally designated as Pichia pastoris were found to represent a novel Komagataella species. Strains of Komagataella mondaviorum sp. nov. UCDFST 71-1024T(type strain) = CBS 15017, UCDFST 54-11.16, UCDFST 54-11.141, UCDFST 68-967.1, and UCDFST 74-1030 were isolated in USA, respectively, from cottonwood tree Populus deltoides in 1971 (Davis, CA), slime flux of Quercus sp. in 1954 (CA), exudate of black oak Q. kelloggii in 1954 (Central Sierra Nevada. CA), dry frass from Salix sp. in 1968 (Soleduck Road, Olympic National Park, WA) and from flux of hackberry tree Celtis sp. in 1974 (CA). The new species was differentiated from Komagataella kurtzmanii, Komagataella pastoris, Komagataella phaffii, Komagataella populi, Komagataella pseudopastoris and Komagataella ulmi by divergence in gene sequences for D1/D2 LSU rRNA, ITS1-5.8S-ITS2, RNA polymerase subunit I and translation elongation factor-1α. Komagataella mondaviorum sp. nov. is registered in MycoBank under MB 821789.

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Acknowledgements

The authors thank Yuriy A. Rybakov, Sergey E. Cheperegin, Erin Cathcart and Russell Fry for technical assistance. This material is based upon work supported by the Russian Foundation for Basic Research under Grant Number 18-54-52002 MHT_a and the National Science Foundation under Grant Number 1349395, which funded, respectively, nuclear genes (EF-1α and RPB1) and ribosomal sequence analyses of yeasts from the Phaff Yeast Culture Collection. Any opinions, findings, and conclusions or recommendations expressed in this material are those of the authors and do not necessarily reflect the views of the National Science Foundation.

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Authors and Affiliations

  1. State Research Institute of Genetics and Selection of Industrial Microorganisms of National Research Centre “Kurchatov Institute”, Moscow, 117545, Russia

    Gennadi I. Naumov & Elena S. Naumova

  2. Phaff Yeast Culture Collection, Department of Food Science and Technology, University of California, Davis, 95616, CA, USA

    Kyria L. Boundy-Mills

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  1. Gennadi I. Naumov
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  2. Elena S. Naumova
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  3. Kyria L. Boundy-Mills
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Correspondence to Gennadi I. Naumov.

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Naumov, G.I., Naumova, E.S. & Boundy-Mills, K.L. Description of Komagataella mondaviorum sp. nov., a new sibling species of Komagataella (Pichia) pastoris. Antonie van Leeuwenhoek 111, 1197–1207 (2018). https://doi.org/10.1007/s10482-018-1028-6

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