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Yamadazyma ubonensis f.a., sp. nov., a novel xylitol-producing yeast species isolated in Thailand

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Abstract

Three hundred and thirty-seven xylose-utilizing yeast strains were isolated from various natural samples. Among these, 68 strains produced xylitol in the range of 0.1–0.69 g xylitol/g xylose. Thirty-nine xylitol-producing strains were identified to be Candida tropicalis. Ten strains were found belonging to 14 known species in the genus Candida, Cyberlindnera, Meyerozyma, Pichia, Wickerhamomyces, Yamadazyma and Cryptococcus. Two strains were identified to be two Candida species and two strains (DMKU-XE142T and DMKU-XE332) were found to be a novel species. Strain DMKU-XE142T was isolated from tree bark and DMKU-XE332 was obtained from decaying plant leaf collected in Thailand. On the basis of morphological, biochemical, physiological and chemotaxonomic characteristics and sequence analysis of the D1/D2 region of the large subunit rRNA gene (LSU) and the internal transcribed spacer (ITS) region, the two strains were determined to represent a novel Yamadazyma species although formation of ascospores was not observed. The sequences of the D1/D2 region of the LSU rRNA gene and the ITS region of the two strains were identical but differed from Yamadazyma phyllophila, the closest species in terms of pairwise sequence similarity of the D1/D2 region, by 1.7 % nucleotide substitutions and 3.5 % nucleotide substitutions in the ITS region. The name Yamadazyma ubonensis f.a., sp. nov. is proposed (type strain is DMKU-XE142T = BCC 61020T = CBS 12859T).

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Acknowledgments

This work was partially supported by the Thailand Graduate Institute of Science and Technology (TGIST) grant TGIST-01-53-038 and the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Commission, Thailand. The authors would like to thanks Ms. Somjit Am-in and Ms. Napatchanok Yuangsaard for their assistance.

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Authors and Affiliations

  1. Department of Microbiology, Faculty of Science, Kasetsart University, Bangkok, 10900, Thailand

    Keeratichaiyanan Junyapate & Savitree Limtong

  2. Bioresources Technology Unit, National Center for Genetic Engineering and Biotechnology (BIOTEC), Pathumthani, Thailand

    Sasitorn Jindamorakot

  3. Center for Advanced Studies in Tropical Natural Resources, National Research University-Kasetsart University, Bangkok, 10900, Thailand

    Savitree Limtong

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  1. Keeratichaiyanan Junyapate
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  2. Sasitorn Jindamorakot
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Correspondence to Savitree Limtong.

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Junyapate, K., Jindamorakot, S. & Limtong, S. Yamadazyma ubonensis f.a., sp. nov., a novel xylitol-producing yeast species isolated in Thailand. Antonie van Leeuwenhoek 105, 471–480 (2014). https://doi.org/10.1007/s10482-013-0098-8

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