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Spathaspora piracicabensis f. a., sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil

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Abstract

Two strains of a novel yeast species were isolated from rotting wood of an ornamental tree (purple quaresmeira, Tibouchina granulosa, Melastomataceae) in an Atlantic Rainforest area in Brazil. Analysis of the sequences of the internal transcribed spacer (ITS-5.8S) region and the D1/D2 domains of the large subunit rRNA gene showed that this species belongs to the Spathaspora clade, and is phylogenetically related to Spathaspora brasiliensis, Candida materiae and Sp. girioi. The novel species ferments D-xylose, producing ethanol, with amounts between 3.37 and 3.48 g L−1 ethanol from 2% d-xylose. Ascospores were not observed from this new species. The name Spathaspora piracicabensis f. a., sp. nov. is proposed to accommodate these isolates. The type strain is UFMG-CM-Y5867T (= CBS 15054T = ESALQ-I54T). The MycoBank number is MB 822,320.

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Funding

This work was funded by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq—Brazil, Grant Numbers 457,499/2014-1 and 160143/2014-4), and Fundação do Amparo a Pesquisa do Estado de Minas Gerais (FAPEMIG, Grant Number APQ-01525-14).

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Authors and Affiliations

  1. Departamento de Ciências Biológicas, Escola Superior de Agricultura Luiz de Queiroz, Universidade de São Paulo, Piracicaba, SP, 13418-900, Brazil

    Camila S. Varize, Lucas D. Lopes, Renata M. Christofoleti-Furlan & Luiz C. Basso

  2. Departamento de Microbiologia, Universidade Federal de Minas Gerais, Belo Horizonte, MG, 31270-901, Brazil

    Raquel M. Cadete & Carlos A. Rosa

  3. Department of Biology, University of Western Ontario, London, ON, N6A 5B7, Canada

    Marc-André Lachance

Authors
  1. Camila S. Varize
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  2. Raquel M. Cadete
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  3. Lucas D. Lopes
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  4. Renata M. Christofoleti-Furlan
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  5. Marc-André Lachance
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  6. Carlos A. Rosa
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  7. Luiz C. Basso
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Corresponding author

Correspondence to Luiz C. Basso.

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10482_2017_974_MOESM1_ESM.pdf

Phylogenetic placement of Spathaspora piracicabensis sp. nov. based on ITS-5.8S region sequences. The tree was constructed by Maximum Likelihood analysis of 379 aligned positions using the Tamura-Nei substitution model with a gamma rate distribution and invariant sites. Bootstraps were determined from 1000 pseudoreplicates and values above 50% are indicated in the tree. Bar, 0.1 substitutions per site. Supplementary material 1 (PDF 87 kb)

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Varize, C.S., Cadete, R.M., Lopes, L.D. et al. Spathaspora piracicabensis f. a., sp. nov., a d-xylose-fermenting yeast species isolated from rotting wood in Brazil. Antonie van Leeuwenhoek 111, 525–531 (2018). https://doi.org/10.1007/s10482-017-0974-8

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  • DOI: https://doi.org/10.1007/s10482-017-0974-8

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