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Silica-Based Phenyl and Octyl Bifunctional Imidazolium as a New Mixed-Mode Stationary Phase for Reversed-Phase and Anion-Exchange Chromatography

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Abstract

In this study, octylbenzimidazolium-modified silica (BeImC8-Sil) was prepared by covalent attachment of 1-octylbenzimidazole to γ-chloropropyl silica. The synthesized materials were characterized by the elemental analysis, IR spectrum, and thermogravimetric analysis. Due to the introduction of phenyl and octyl groups on the quaternary imidazolium, the developed BeImC8-Sil column can function via both reversed-phase and anion-exchange retention mechanisms. The chromatographic properties of the synthesized material were investigated by the separations of polycyclic aromatic hydrocarbons, mono-substituted derivatives of benzene, anilines, and phenols, revealing the existence of multiple interactions, including hydrogen bonding, π–π stacking, electrostatic forces, and hydrophobic interactions in reversed-phase mode; inorganic and organic anions were also separated mainly through anion-exchange interaction. The proposed BeImC8-Sil is a promising mixed-mode stationary phase for the separation of complex samples in high-performance liquid chromatography.

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Acknowledgments

The authors express their thanks to the support of the “Hundred Talents Program” of the Chinese Academy of Sciences, and the National Natural Science Foundation of China (No. 21275133).

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Authors and Affiliations

  1. Key Laboratory of Chemical Biology and Organic Chemistry of Henan, Chemistry Department, Zhengzhou University, Zhengzhou, 450052, China

    Qiong Jiang, Wenjie Zhao & Shusheng Zhang

  2. Key Laboratory for Natural Medicine of Gansu Province, Lanzhou Institute of Chemical Physics, Chinese Academy of Sciences, Lanzhou, 730000, China

    Qiong Jiang & Hongdeng Qiu

  3. School of Chemistry and Chemical Engineering, Henan University of Technology, Zhengzhou, 450001, China

    Wenjie Zhao

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  1. Qiong Jiang
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  2. Wenjie Zhao
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Correspondence to Hongdeng Qiu or Shusheng Zhang.

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Jiang, Q., Zhao, W., Qiu, H. et al. Silica-Based Phenyl and Octyl Bifunctional Imidazolium as a New Mixed-Mode Stationary Phase for Reversed-Phase and Anion-Exchange Chromatography. Chromatographia 79, 1437–1443 (2016). https://doi.org/10.1007/s10337-016-3166-1

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  • DOI: https://doi.org/10.1007/s10337-016-3166-1

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