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Novel imidazolium-embedded N,N-dimethylaminopropyl-functionalized silica-based stationary phase for hydrophilic interaction/reversed-phase mixed-mode chromatography

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Abstract

A novel imidazolium-embedded N,N-dimethylaminopropyl-functionalized silica-based stationary phase (Sil-ImCl) was prepared and further used for hydrophilic interaction/reversed-phase mixed-mode chromatography. The Sil-ImCl stationary phase was respectively characterized by Fourier transform infrared spectrometry, thermogravimetric analysis, and element analysis. A variety of hydrophilic or hydrophobic compounds were used to evaluate the retention mechanisms of the developed stationary phase, and the effects of buffer salt concentration and pH of mobile phase on the retention of these compounds were also investigated. The developed stationary phase was successfully applied for separation of nucleosides and nucleic acid bases, water-soluble vitamins, phenols, and positional isomers. Moreover, simultaneous separation of polar and nonpolar compounds was also achieved with high resolution, outperforming the commercially available C8 column and amino column. Furthermore, the Sil-ImCl stationary phase has been successfully applied for separation of secondary metabolites of Hansfordia sinuosae. All these results demonstrate that the Sil-ImCl stationary phase might be promising for separation of complex polar and nonpolar compounds with high efficiency, especially in biological industry.

Novel imidazolium-embedded N,N-dimethylaminopropyl-functionalized silica-based stationary phase for HILIC/RPLC mixed-mode chromatography

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Acknowledgments

We are grateful for the financial support from National Natural Science Foundation of China (21205027), Natural Science Foundation of Hebei Province (B2015201016), the Young Talent Program in College and University of Hebei Province (BJ2014005), and Natural Science Foundation of Hebei University (2011–225).

Conflict of interest

The authors have declared that no conflict of interest exists.

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Authors and Affiliations

  1. Key Laboratory of Medicinal Chemistry and Molecular Diagnosis, Ministry of Education and Key Laboratory of Pharmaceutical Quality Control of Hebei Province, College of Pharmaceutical Sciences, Hebei University, Baoding, 071002, China

    Shijia Liu, Hongxin Xu, Jiaojiao Yu, Danyang Li, Mingyan Li, Xiaoqiang Qiao & Hongyuan Yan

  2. College of Chemistry & Environmental Science, Hebei University, Baoding, 071002, China

    Xinying Qin

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  1. Shijia Liu
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  2. Hongxin Xu
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Correspondence to Xiaoqiang Qiao.

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Liu, S., Xu, H., Yu, J. et al. Novel imidazolium-embedded N,N-dimethylaminopropyl-functionalized silica-based stationary phase for hydrophilic interaction/reversed-phase mixed-mode chromatography. Anal Bioanal Chem 407, 8989–8997 (2015). https://doi.org/10.1007/s00216-015-9064-9

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  • DOI: https://doi.org/10.1007/s00216-015-9064-9

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