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Two new species, Phytophthora nagaii sp. nov. and P. fragariaefolia sp. nov., causing serious diseases on rose and strawberry plants, respectively, in Japan

  • Fungal Diseases
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Abstract

A new disease of rose was noticed in Chiba Prefecture of Japan in 1968, and the pathogen was initially identified as Phytophthora megasperma based on morphological characteristics. Similar Phytophthora isolates have since been collected from rose plants in Chiba, Kanagawa, and Shizuoka Prefectures. In 2005, several Phytophthora isolates were recovered from crowns of strawberry plants in Hokkaido Prefecture. These were considered to be members of a new species. In this study, we re-examined all these isolates using morphological and physiological studies and a multilocus phylogenetic analysis. The rose and strawberry isolates were mostly similar morphologically and physiologically, with some exceptions. The rose isolates differed significantly from P. megasperma sensu stricto and other related Phytophthora species. The rose and strawberry isolates had external proliferation of sporangia, characteristic funnel-shaped oogonia, predominantly paragynous antheridia, and fast growth rates of 10.5 mm/24 h at an optimum temperature of 28 °C. In the multilocus phylogenetic tree constructed using sequences from the rDNA ITS regions, rDNA LSU, and the translation elongation factor 1-α, β-tubulin and coxI genes, they formed a distinct monophyletic group in clade 7 with strong bootstrap support. The rose and strawberry isolates separated into two distinct groups. The results indicate that the rose and strawberry isolates constitute two separate species, designated here as Phytophthora nagaii and P. fragariaefolia.

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Acknowledgments

We thank Drs. Yuji Nagai, E. M. Hansen, and Tsuneo Watanabe for kind suggestions.

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Authors and Affiliations

  1. The United Graduate School of Agricultural Science, Gifu University, Gifu, 501-1193, Japan

    Mohammad Ziaur Rahman

  2. Chiba Prefectural Agriculture and Forestry Research Center, Southern Horticultural Institute, Tateyama, Chiba, 294-0014, Japan

    Seiji Uematsu

  3. Hokkaido Central Agricultural Experiment Station, Hokkaido, 069-1395, Japan

    Toru Takeuchi & Kayo Shirai

  4. Life Science Research Centre, Gifu University, Gifu, 501-1193, Japan

    Haruhisa Suga

  5. River Basin Research Center, Gifu University, Gifu, 501-1193, Japan

    Yasushi Ishiguro & Koji Kageyama

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  1. Mohammad Ziaur Rahman
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  2. Seiji Uematsu
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  3. Toru Takeuchi
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  4. Kayo Shirai
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  5. Yasushi Ishiguro
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  6. Haruhisa Suga
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  7. Koji Kageyama
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Correspondence to Mohammad Ziaur Rahman.

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Rahman, M.Z., Uematsu, S., Takeuchi, T. et al. Two new species, Phytophthora nagaii sp. nov. and P. fragariaefolia sp. nov., causing serious diseases on rose and strawberry plants, respectively, in Japan. J Gen Plant Pathol 80, 348–365 (2014). https://doi.org/10.1007/s10327-014-0519-1

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  • DOI: https://doi.org/10.1007/s10327-014-0519-1

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