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Optimization of lipase production by Staphylococcus warneri EX17 using the polydimethylsiloxanes artificial oxygen carriers

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Journal of Industrial Microbiology & Biotechnology

Abstract

In this research, the combined effects of polydimethylsiloxane (PDMS) and different conditions of oxygen volumetric mass transfer coefficient (kLa) on lipase production by Staphylococcus warneri EX17 were studied and optimized in bioreactor cultures. Raw glycerol from biodiesel synthesis was used as the sole carbon source. Full-factorial central composite design and the response surface methodology were employed for the experimental design and analysis of the results. The optimal polydimethylsiloxane concentration and mass coefficient transfer (kLa) were found to be 13.5% (v/v) and 181 h−1, respectively. Under these conditions, the maximal cell production obtained was 10.0 g/l, and the volumetric lipase activities of approximately 490 U/l, after 6 h of cultivation. These results are in close agreement with the model predictions. Results obtained in this work reveal the positive effects of PDMS on oxygen volumetric mass transfer coefficient (kLa) in the Staphylococcus warneri EX17 cultivation and lipase production.

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Acknowledgments

The authors wish to thank The Brazilian Bureau for Science & Technology (CNPQ) and CAPES for their financial support of this project and for the scholarship to the first author.

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Authors and Affiliations

  1. Food Science & Technology Institute, Federal University of Rio Grande do Sul, Av. Bento Goncalves, 9500, PO Box 15090, Porto Alegre, RS, 91501-970, Brazil

    Fernanda Roberta Rech & Marco Antônio Záchia Ayub

  2. Institute of Education, Science & Technology of Rio Grande do Sul, Rua Ramiro Barcelos, Porto Alegre, RS, 2777, Brazil

    Giandra Volpato

Authors
  1. Fernanda Roberta Rech
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  2. Giandra Volpato
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  3. Marco Antônio Záchia Ayub
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Correspondence to Marco Antônio Záchia Ayub.

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Rech, F.R., Volpato, G. & Ayub, M.A.Z. Optimization of lipase production by Staphylococcus warneri EX17 using the polydimethylsiloxanes artificial oxygen carriers. J Ind Microbiol Biotechnol 38, 1599–1604 (2011). https://doi.org/10.1007/s10295-011-0950-7

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  • DOI: https://doi.org/10.1007/s10295-011-0950-7

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