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Enhanced oxygen transfer rates in fermentation using soybean oil-in-water dispersions

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Summary

The effect of soybean oil on the volumetric oxygen transfer coefficient during the cultivation ofAerobacter aerogenes cells is presented. For our aeration-agitation conditions (0.278 vvm and 500 rpm), it has been demonstrated that the use 19% (v/v) of soybean oil enabled a 1.85-fold increase of thek l a coefficient (calculated on a per liter aqueous phase basis). For smaller volumetric oil fractions,k L a increased linearly with the oil loading. Because of the oxygen-vector properties of soybean oil, this oil is able to significantly increase thek L a of a bioreactor.

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Abbreviations

C*, C:

saturation and actual dissolved oxygen concentrations respectively (g/m3)

KLa:

volumetric oxygen transfer coefficient (h−1)

KLainitial :

k La measured before the oil addition (h−1)

MO2 :

molar mass of oxygen (dalton)

N:

oxygen transfer rate (g/m3. h)

PO2. PN2 :

partial pressures ofO 2 andN 2 in the gas (atm)

PH2OT :

partial pressure of water in air at the temperatureT (atm)

PT :

total pressure (atm)

Q0 :

volumetric flow rate of outlet air before seeding (m3/h)

Sp:

spreading coefficient (dynes/cm)

T:

absolute temperature of outlet gas (K)

Vi :

volume of the liquidi in the fermentor (m3)

VM :

molar volume at 273 K and 1 atm (m3/mole)

σij :

interfacial tension betweeni andj componants (dynes/cm)

∈v :

volumetric fraction of the oil (v/v)

G:

gas

O:

oil

W:

water

i:

inlet

o:

outlet

References

  • Adler, I., Diekmann, J., Hartke, W., Hecht, V., Rohn, F., andSchugerl, K. (1980). Eur. J. Appl. Microbiol. Biotechnol. 10, 171–186.

    Google Scholar 

  • Bader, F.G., Boekeloo, M.K., Graham, H.E., andCagle, J.W. (1984). Biotechnol. Bioeng. 26, 848–856.

    Google Scholar 

  • Eiki, H., Gushima, H., Saito, T., Ishida, H., Oka, Y., andOsono, T. (1988). J. Ferment. Technol. 66, 559–565.

    Google Scholar 

  • Ettler, P. (1987). Acta Biotechnol. 7, 3–8.

    Google Scholar 

  • Huber, F.M., andTietz, A.J. (1983). Biotechnol. Lett. 5, 385–390.

    Google Scholar 

  • Kralovcova, E., Krumphanzl, V., andVanek, Z. (1984). Folia Microbiol. 29, 35–42.

    Google Scholar 

  • Leuchtenberger, A., andRuttloff, H. (1985). Acta Biotechnol. 5, 293–296.

    Google Scholar 

  • Linek, V., andBenes, P. (1976). Chem. Engng. Sci. 31, 1037–1046.

    Google Scholar 

  • Mc Millan, J.D., andWang, D.I.C. (1987). Ann. N. Y. Acad. Sci. USA 506, 569–582.

    Google Scholar 

  • Mc Millan, J.D., andWang, D.I.C. (1990). Ann. N. Y. Acad. Sci. USA 589, 283–300.

    Google Scholar 

  • Motkova, M.O., Drobysheva, T.N., Gladkikh, E.G., andKorobkova, T.P. (1983). Antibiotiki 28, 814–818.

    Google Scholar 

  • Mozes, M., andRouxhet, P.G. (1987). J. Microbiol. Methods 6, 99–112.

    Google Scholar 

  • Prochazka, P., Nohynek, M., Vanek, Z., andRokos, J. (1983). Folia Microbiol. 28, 406–408.

    Google Scholar 

  • Ramachandran, K.B., andGoma, G. (1988). J. Biotechnol. 9, 39–46.

    Google Scholar 

  • Rezanka, T., Vanek, Z., Klanova, K., andPodojil, M. (1984). Folia Microbiol. 29, 306–309.

    Google Scholar 

  • Rols, J.L., Condoret, J.S., Fonade, C., andGorna, G. (1990). Biotechnol. Bioeng. 35, 427–435.

    Google Scholar 

  • Rols, J.L., andGoma, G. (1989). Biotechnol. Adv. 7, 1–14.

    Google Scholar 

  • Rosenberg, M., Gutnick, D., andRosenberg, E. (1980). FEMS Microbiol. Lett. 9, 29–33.

    Google Scholar 

  • Ryu, D.D.Y., andHospodka, J. (1980). Biotechnol. Bioeng. 22, 289–298.

    Google Scholar 

  • Sablayrolles, J.M., andGoma, G. (1984). Biotechnol. Bioeng. 26, 148–155.

    Google Scholar 

  • Stowell, J.D. (1987). The application of oils and fats in antibiotic processes, in “Carbon substrates in biotechnology”, J.D. Stowell, A.J. Beardsmore, C.W. Keevil and J.R. Woodward, eds, Society for Gereral Microbiology, 21, 139–159, IRL Press.

    Google Scholar 

  • Surikova, E.I., andKalinina, M.V. (1969). Mikrobiologiya 37, 152–158.

    Google Scholar 

  • Vardar-Sukan, F. (1988). J. Chem. Tech. Biotechnol. 43, 39–47.

    Google Scholar 

  • Voigt, J., andSchugerl, K. (1981). Eur. J. Appl. Microbiol. Biotechnol. 11, 97–105.

    Google Scholar 

  • Winkler, L.W.Z. (1982). Physik. Chem. 9, 173.

    Google Scholar 

Download references

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Authors and Affiliations

  1. Department de Génie Biochimique et Alimentaire, UA CNRS 544, Institut National des Sciences Appliquées, Complexe Scientifique de Rangueil, 31077, Toulouse Cédex, France

    J. L. Rols & G. Goma

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  1. J. L. Rols
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  2. G. Goma
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Rols, J.L., Goma, G. Enhanced oxygen transfer rates in fermentation using soybean oil-in-water dispersions. Biotechnol Lett 13, 7–12 (1991). https://doi.org/10.1007/BF01033508

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