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Development and application of co-culture for ethanol production by co-fermentation of glucose and xylose: a systematic review

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Journal of Industrial Microbiology & Biotechnology

Abstract

This article reviews current co-culture systems for fermenting mixtures of glucose and xylose to ethanol. Thirty-five co-culture systems that ferment either synthetic glucose and xylose mixture or various biomass hydrolysates are examined. Strain combinations, fermentation modes and conditions, and fermentation performance for these co-culture systems are compared and discussed. It is noted that the combination of Pichia stipitis with Saccharomyces cerevisiae or its respiratory-deficient mutant is most commonly used. One of the best results for fermentation of glucose and xylose mixture is achieved by using co-culture of immobilized Zymomonas mobilis and free cells of P. stipitis, giving volumetric ethanol production of 1.277 g/l/h and ethanol yield of 0.49–0.50 g/g. The review discloses that, as a strategy for efficient conversion of glucose and xylose, co-culture fermentation for ethanol production from lignocellulosic biomass can increase ethanol yield and production rate, shorten fermentation time, and reduce process costs, and it is a promising technology although immature.

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Acknowledgments

This work is supported by the Graduate Research Scholars Program (GRSP) of the Alabama Experimental Program to Stimulate Competitive Research (EPSCoR). Three anonymous referees and the journal editor, Dr. Thomas W. Jeffries, are gratefully acknowledged for their insightful suggestions and valuable comments. Dr. Fred L. Strickland, Dr. Heping Liu, Dr. Shirlaine Koh and Mr. Ronald Putt are highly appreciated for their helpful suggested revisions.

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    Yanli Chen

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Chen, Y. Development and application of co-culture for ethanol production by co-fermentation of glucose and xylose: a systematic review. J Ind Microbiol Biotechnol 38, 581–597 (2011). https://doi.org/10.1007/s10295-010-0894-3

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