Abstract
Substrates that contain hexose as well as pentose sugars can form an interesting substrate for the production of ethanol. Pichia stipitis and a respiratory-deficient mutant of Saccharomyces diastaticus were used to convert such a substrate into ethanol under continuous culture conditions. With a sugar mixture (glucose 70%/xylose 30%) at 50 g/l, the xylose was entirely consumed when the dilution rate (D) did not exceed 0.006 h−1 whereas the glucose was entirely consumed whatever the D. The study of influence of initial substrate concentration (S0) was performed at D = 0.015 h−1. Under these conditions the substrate was entirely consumed when its initial concentration did not exceed 20 g/l. With S0 = 80 g/l the residual xylose concentration reached 20.5 g/l. At low D or at low S0, P. stipitis was the dominant species in the fermentor. Increasing the D or S0 resulted in the wash-out of P. stipitis mainly because of its low ethanol tolerance.
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Correspondence to: J. P. Delgenes
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Laplace, J.M., Delgenes, J.P., Moletta, R. et al. Effects of culture conditions on the co-fermentation of a glucose and xylose mixture to ethanol by a mutant of Saccharomyces diastaticus associated with Pichia stipitis . Appl Microbiol Biotechnol 39, 760–763 (1993). https://doi.org/10.1007/BF00164463
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DOI: https://doi.org/10.1007/BF00164463