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Isolation and characterization of the gene encoding a manganese peroxidase from Lentinula edodes

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Mycoscience

Abstract

A genomic DNA sequence and cDNA encoding a putative manganese peroxidase were isolated from the white-rot basidiomycete Lentinula edodes. The gene, called lemnp1, consists of a 1985-bp open reading frame interrupted by 16 introns and was flanked by an upstream region having putative CAAT, TATA, and heat shock elements and by a downstream region having polyadenylation signals. The lemnp1 gene encodes a protein of 364 amino acids that shows high sequence homology to manganese peroxidases of other basidiomycetes. The deduced N-terminal amino acid sequence is different from the L. edodes manganese peroxidase reported previously.

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Author notes

  1. Masaru Nagai

    Present address: Institute for Environmental Sciences, Japan

Authors and Affiliations

  1. Iwate Biotechnology Research Center, 22-174-4 Narita, Kitakami, Iwate, 024-0003, Japan

    Masaru Nagai, Yuichi Sakamoto, Keiko Nakade & Toshitsugu Sato

Authors
  1. Masaru Nagai
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  2. Yuichi Sakamoto
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  3. Keiko Nakade
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  4. Toshitsugu Sato
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Corresponding author

Correspondence to Toshitsugu Sato.

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Nagai, M., Sakamoto, Y., Nakade, K. et al. Isolation and characterization of the gene encoding a manganese peroxidase from Lentinula edodes . Mycoscience 48, 125–130 (2007). https://doi.org/10.1007/s10267-006-0334-4

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  • DOI: https://doi.org/10.1007/s10267-006-0334-4

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