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High-yield production of manganese peroxidase, lignin peroxidase, and versatile peroxidase in Phanerochaete chrysosporium

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An Erratum to this article was published on 19 October 2014

Abstract

The white-rot fungus Phanerochaete chrysosporium secretes extracellular oxidative enzymes during secondary metabolism, but lacks versatile peroxidase, an enzyme important in ligninolysis and diverse biotechnology processes. In this study, we report the genetic modification of a P. chrysosporium strain capable of co-expressing two endogenous genes constitutively, manganese peroxidase (mnp1) and lignin peroxidase (lipH8), and the codon-optimized vpl2 gene from Pleurotus eryngii. For this purpose, we employed a highly efficient transformation method based on the use of shock waves developed by our group. The expression of recombinant genes was verified by PCR, Southern blot, quantitative real-time PCR (qRT-PCR), and assays of enzymatic activity. The production yield of ligninolytic enzymes was up to four times higher in comparison to previously published reports. These results may represent significant progress toward the stable production of ligninolytic enzymes and the development of an effective fungal strain with promising biotechnological applications.

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Acknowledgments

NCL and DMO are indebted to CONACYT for doctoral fellowships 219893 and 219950.

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Authors and Affiliations

  1. Centro de Investigación y de Estudios Avanzados del IPN, Unidad Irapuato, Km. 9.6 Carretera Irapuato-León, 36821, Irapuato, Gto, Mexico

    Nancy Coconi-Linares, Denis Magaña-Ortíz, Doralinda A. Guzmán-Ortiz & Miguel A. Gómez-Lim

  2. Centro de Física Aplicada y Tecnología Avanzada, Universidad Nacional Autónoma de México, A.P. 1-1010, 76000, Querétaro, Qro, Mexico

    Francisco Fernández & Achim M. Loske

Authors
  1. Nancy Coconi-Linares
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  2. Denis Magaña-Ortíz
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  3. Doralinda A. Guzmán-Ortiz
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  4. Francisco Fernández
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  5. Achim M. Loske
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  6. Miguel A. Gómez-Lim
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Correspondence to Miguel A. Gómez-Lim.

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Coconi-Linares, N., Magaña-Ortíz, D., Guzmán-Ortiz, D.A. et al. High-yield production of manganese peroxidase, lignin peroxidase, and versatile peroxidase in Phanerochaete chrysosporium . Appl Microbiol Biotechnol 98, 9283–9294 (2014). https://doi.org/10.1007/s00253-014-6105-9

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