Abstract
Colorful shells in bivalves are mostly caused by the presence of biological pigments, among which melanin is a key component in the formation of shell colours. Cyclic adenosine monophosphate (cAMP) is an important messenger in the regulation of pigmentation in some species. However, the role of cAMP in bivalve melanogenesis has not yet been reported. In this study, we performed in vitro and in vivo experiments to determine the role of cAMP in regulating melanogenesis in Pacific oysters. Besides, the function of cAMP-responsive element modulator (CREM) and the interactions between CREM and melanogenic genes were investigated. Our results showed that a high level of cAMP promotes the expression of melanogenic genes in Pacific oysters. CREM controls the expression of the MITF gene under cAMP regulation. In addition, CREM can regulate melanogenic gene expression, tyrosine metabolism, and melanin synthesis. These results indicate that cAMP plays an important role in the regulation of melanogenesis in Pacific oysters. CREM is a key transcription factor in the oyster melanin synthesis pathway, which plays a crucial role in oyster melanin synthesis through a cAMP-mediated CREM-MITF-TYR axis.
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The datasets generated in the current study are available from the corresponding author on reasonable request.
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Acknowledgements
We thank the grants from the National Key R&D Program of China (2022YFD2400305), Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province (2022LZGCQY010, 2022TZXD002 and 2021ZLGX03), and China Agriculture Research System Project (CARS-49).
Funding
This work was supported by grants from the National Key R&D Program of China (2022YFD2400305), Earmarked Fund for Agriculture Seed Improvement Project of Shandong Province (2022LZGCQY010, 2022TZXD002 and 2021ZLGX03), and China Agriculture Research System Project (CARS-49).
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Kunyin Jiang: Completion of the experiment, data analysis, and Writing—original draft. Qi Li: Experimental design and coordination, funding acquisition, and Writing—review & editing. Hong Yu: Resources. Lingfeng Kong: Resources. Shikai Liu: Investigation.
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10126_2024_10309_MOESM1_ESM.pdf
Fig S1. Flowchart of RNAi experiment. The BRLZ DNA binding domain was selected as the target for this experiment (~500 bp). Double-stranded RNA (dsRNA of CREM) was synthesized by in vitro transcription and verified through 1% agarose gel electrophoresis. Forty oysters were injected with 60 μg dsRNA (40 μL) each time. A total of seven injections were performed at an interval of 48 h. (PDF 3783 KB)
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Jiang, K., Yu, H., Kong, L. et al. cAMP-Mediated CREM-MITF-TYR Axis Regulates Melanin Synthesis in Pacific Oysters. Mar Biotechnol (2024). https://doi.org/10.1007/s10126-024-10309-9
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DOI: https://doi.org/10.1007/s10126-024-10309-9