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Comparison of Protein Expression Profiles of the Hepatopancreas in Fenneropenaeus chinensis Challenged with Heat-inactivated Vibrio anguillarum and White Spot Syndrome Virus

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Abstract

Fenneropenaeus chinensis (Chinese shrimp) culture industry, like other Penaeidae culture, has been seriously affected by the shrimp diseases caused by bacteria and virus. To better understand the mechanism of immune response of shrimp to different pathogens, proteome research approach was utilized in this study. Firstly, the soluble hepatopancreas protein samples in adult Chinese shrimp among control, heat-inactivated Vibrio-challenged and white spot syndrome virus-infected groups were separated by 2-DE (pH range, 4–7; sodium dodecyl sulfate polyacrylamide gel electrophoresis (SDS-PAGE) and pH range, 3–10; tricine-SDS-PAGE). Then the differentially expressed protein spots (≥1.5-fold or ≤0.67-fold averagely of controls) were analyzed by LC-ESI-MS/MS. Using Mascot online database searching algorithm and SEQUEST searching program, 48 and 49 differentially expressed protein spots were successfully identified in response to Vibrio and white spot syndrome virus infection, respectively. Based on these results, we discussed the mechanism of immune response of the shrimp and shed light on the differences between immune response of shrimp toward Vibrio and white spot syndrome virus. This study also set a basis for further analyses of some key genes in immune response of Chinese shrimp.

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Abbreviations

FcDB:

Fenneropenaeus chinensis peptides database

PDI:

Protein disulfide isomerase

ROS:

Reactive oxygen species

WSSV:

White spot syndrome virus

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Acknowledgments

This work was financially supported by Major State Basic Research Development Program of China (973 program) (2012CB114403) and National Natural Science Foundation of China (41076101).

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Authors and Affiliations

  1. Key Laboratory of Experimental Marine Biology, Institute of Oceanology, Chinese Academy of Sciences, Qingdao, 266071, China

    Hao Jiang, Fuhua Li, Jiquan Zhang, Jinkang Zhang, Bingxin Huang, Yang Yu & Jianhai Xiang

  2. University of Chinese Academy of Sciences, Beijing, 100049, China

    Hao Jiang, Jinkang Zhang & Yang Yu

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  1. Hao Jiang
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  2. Fuhua Li
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Correspondence to Jianhai Xiang.

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Jiang, H., Li, F., Zhang, J. et al. Comparison of Protein Expression Profiles of the Hepatopancreas in Fenneropenaeus chinensis Challenged with Heat-inactivated Vibrio anguillarum and White Spot Syndrome Virus. Mar Biotechnol 16, 111–123 (2014). https://doi.org/10.1007/s10126-013-9538-8

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