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Proteomic analysis of differentially expressed proteins in the lymphoid organ of Vibrio harveyi-infected Penaeus monodon

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Abstract

The protein expression profiles of the lymphoid organ, taken from mock and systemic Vibrio harveyi-infected Penaeus monodon at 6 and 48 h post infection, were revealed. The considerable changes in the expression level of several proteins were observed between the mock and V. harveyi-infected shrimps. From 30 analyzed protein spots with 27 differentially expressed, 21 were known proteins with the most common of these being cytoskeleton proteins (33%) which were all down-regulated upon systemic bacterial infection. Other six proteins including four proteins that are involved in the shrimp immunity (alpha-2-macroglobulin, transglutaminase, heat shock protein 1 and hemocyanin subunit Y), and two proteins that are involved in metabolism (triosephosphate isomerase) and cell signaling (14-3-3 like protein), displayed significantly decreased expression levels. There was, however, an increase in the expression level of the ATP synthase beta subunit, a protein involved in energy balance. Transcription levels of ATP synthase beta subunit and 14-3-3 like protein were up- and down-regulated, respectively, in accord with the observed protein expression levels, but the alpha-2-macroglobulin transcript levels were significantly increased in contrast to the decreased protein expression levels. Interestingly, partial gene silencing of ATP synthase beta subunit revealed a high cumulative mortality of the knockdown shrimps (73.3%) and a dramatic reduction of the total hemocyte numbers in the survival shrimps. These altered proteins are likely to play essential roles in shrimp defense against the pathogenic bacterium V. harveyi.

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Acknowledgments

This work was supported by research grants from the Thailand National Center for Genetic Engineering and Biotechnology (BIOTEC) and the Higher Education Research Promotion and National Research University Project of Thailand, Office of the Higher Education Commission (FW643A). Equipments are provided for by the Thai Government Stimulus Package 2 (TKK2555) under the Project for Establishment of Comprehensive Center for Innovative Food, Health Products and Agriculture. This work was also financially supported by grants from the Office of the Higher Education Commission and the Thailand Research Fund (TRF) to Kunlaya Somboonwiwat (Grant No. MRG5280119). Support from Chulalongkorn University, through the Ratchadaphisek Somphot Endowment Grants for Development of New Faculty Staff, the Faculty of Science, Chulalongkorn University (Grant No. RESA1B1-11) is acknowledged. Vorrapon Chaikeeratisak expresses his gratitude to the TRF for the fellowship under the Royal Golden Jubilee Ph.D. program.

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Authors and Affiliations

  1. Center of Excellence for Molecular Biology and Genomics of Shrimp, Department of Biochemistry, Faculty of Science, Chulalongkorn University, 254 Phayathai Road, Bangkok, 10330, Thailand

    Vorrapon Chaikeeratisak, Kunlaya Somboonwiwat & Anchalee Tassanakajon

  2. Institute of Biological Chemistry, Academia Sinica, Taipei, Taiwan

    Hao-Ching Wang

  3. Institute of Zoology, College of Life Science, National Taiwan University, Taipei, Taiwan, ROC

    Chu Fang Lo

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  1. Vorrapon Chaikeeratisak
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  2. Kunlaya Somboonwiwat
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  3. Hao-Ching Wang
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  4. Chu Fang Lo
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  5. Anchalee Tassanakajon
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Correspondence to Anchalee Tassanakajon.

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Chaikeeratisak, V., Somboonwiwat, K., Wang, HC. et al. Proteomic analysis of differentially expressed proteins in the lymphoid organ of Vibrio harveyi-infected Penaeus monodon . Mol Biol Rep 39, 6367–6377 (2012). https://doi.org/10.1007/s11033-012-1458-6

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  • DOI: https://doi.org/10.1007/s11033-012-1458-6

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