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Enhancing nerve regeneration in infraorbital nerve injury rat model: effects of vitamin B complex and photobiomodulation

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Abstract

Orofacial nerve injuries may result in temporary or long-term loss of sensory function and decreased quality of life in patients. B vitamins are required for DNA synthesis and the repair and maintenance of phospholipids. In particular, vitamins B1, B6, and B12 are essential for neuronal function. Deficiency in vitamin B complex (VBC) has been linked to increased oxidative stress, inflammation and demyelination. Photobiomodulation (PBM) has antioxidant activity and is neuroprotective. In addition, a growing literature attests to the positive effects of PBM on nerve repair. To assess the effect of PBM and VBC on regenerative process we evaluated the expression of brain-derived neurotrophic factor (BDNF), nerve growth factor (NGF), myelin basic protein (MBP), laminin and neurofilaments (NFs) using Western blotting to identify regenerative pattern after chronic constriction injury of the infraorbital nerve (CCI IoN) treated by PBM, VBC or its combination. After CCI IoN, the rats were divided into six groups naive, sham, injured (CCI IoN), treated with photobiomodulation (904 nm, 6.23 J/cm2, CCI IoN + PBM), treated with VBC (containing B1, B6 and B12) 5 times, CCI IoN + VBC) and treated with PBM and VBC (CCI IoN + VBC + PBM). The treatments could revert low expression of BDNF, MBP and laminin. Also reverted the higher expression of neurofilaments and enhanced expression of NGF. PBM and VBC could accelerate injured infraorbital nerve repair in rats through reducing the expression of neurofilaments, increasing the expression of BDNF, laminin and MBP and overexpressing NGF. These data support the notion that the use of PBM and VBC may help in the treatment of nerve injuries. This finding has potential clinical applications.

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Acknowledgements

Martins D.O., was the recipient of a FAPESP Postdoctoral Scholarship (2015/24256-0).

Funding

This study was financially supported by the Brazilian funding agency, Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP), Grant number—2015/24256–0; 2014/24533–0; 2017/05218–5, and by Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq), Grant number—157284/2017–4; 405853/2018–1. The funding agencies play no role in the design of the study, data collection, analysis, interpretation of the data, or in writing the manuscript.

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Authors and Affiliations

  1. Division of Neuroscience/Hospital Sírio-Libânes, Street Daher Cutait 69, São Paulo, SP, 01308-060, Brazil

    Daniel Oliveira Martins

  2. Departmento de Anatomia, Laboratory of Functional Neuroanatomy of Pain, Universidade de São Paulo Instituto de Ciências Biomédicas, São Paulo, SP, Brazil

    Daniel Oliveira Martins, Daniel Pereira Marques & Marucia Chacur

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  1. Daniel Oliveira Martins
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Correspondence to Daniel Oliveira Martins.

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The study was approved by the local committees on ethics on the use of animals (protocol number 3872071118), Universidade de São Paulo Instituto de Ciências Biomédicas, SP, Brazil.

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Martins, D.O., Marques, D.P. & Chacur, M. Enhancing nerve regeneration in infraorbital nerve injury rat model: effects of vitamin B complex and photobiomodulation. Lasers Med Sci 39, 119 (2024). https://doi.org/10.1007/s10103-024-04067-2

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