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Effect of light-emitting diodes, platelet-rich plasma, and their combination on the activity of sheep tenocytes

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Abstract

Healthy tendons play an important role in joint movements and subjected to a group of pathologies called tendinopathy due to multiple factors. Tendons have a slowly repairing process due to the low vascularity and cellularity. Treatment options aimed at potentiating the healing response and relieving symptoms. Phototherapy and platelet-rich plasma were novel treatment modalities in tendons based on photobiomodulation and growth factors during healing, and the results were encouraging suggesting calibrating treatment parameters. This study utilizes cell culture to explore the potential effect of light-emitting diode and/or growth factors in the form of platelet-rich plasma (PRP) on the activity of tenocytes isolated from sheep Achilles tendons by measuring the cell metabolism and cell mobility using cell viability and migration assays to proof safety and confirm activity. Results showed that sheep tenocyte-cultured groups treated with 5% platelet-rich plasma alone or combined with 4 J/cm2 light-emitting diode have increased viability significantly when compared to control group after a 48 h, while light-emitting diode treatment has not decreased cell migration significantly when compared with control. Result suggests that using platelet-rich plasma alone or combined with light-emitting diode might have potential to enhance healing response at the conditions applied. PRP could enhance proliferation while LED could enhance migration and proliferation. Further research is needed at longer durations.

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Acknowledgments

J. A. M. Alzyoud is grateful to Hamdi Mango Centre for Scientific Research, The University of Jordan, Amman, Jordan, for their support.

Funding

This research is partially supported by Hamdi Mango Centre for Scientific Research, The University of Jordan, Amman, Jordan.

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Authors and Affiliations

  1. Faculty of Allied Health Sciences, The Hashemite University, Zarqa, Jordan

    Jihad A. M. Alzyoud, Saleh A. Al Najjar, Seham Talat & Abd Al-Rahman S. Al-Shudiefat

  2. School of Pharmacy, The University of Jordan, Amman, Jordan

    Basha’er Abu-Irmaileh & Yasser Bustanji

  3. Hamdi Mango Centre for Scientific Research, The University of Jordan, Amman, Jordan

    Yasser Bustanji

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  1. Jihad A. M. Alzyoud
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  2. Saleh A. Al Najjar
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Alzyoud, J.A.M., Al Najjar, S.A., Talat, S. et al. Effect of light-emitting diodes, platelet-rich plasma, and their combination on the activity of sheep tenocytes. Lasers Med Sci 34, 759–766 (2019). https://doi.org/10.1007/s10103-018-2657-8

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