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Comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis

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Abstract

Fatty acids are one of the most important nutrients in food. Acid- or base-catalyzed transesterification methods are commonly used for the analysis of fatty acids. However, several drawbacks were reported for these methods, including the isomerization and degradation of fatty acids. Lipase-catalyzed reactions are usually undertaken at mild conditions, preventing such problems. In this study, commercial resin-bound lipase from Candida antartica was tested for possible application in fatty acid methyl ester analysis. Experimental parameters, including temperature, reaction time, and re-cycling were evaluated. The optimized condition was (5-10 mg lipid, 0.5 mL of MeOH, and 50 mg Novozyme 435 in 2 mL toluene, 80°C for 1 h). In optimized condition, the lipase-catalyzed methods yielded similar results with the chemical method. In overall, lipase-catalyzed transesterification can be a useful alternative to acid-catalyzed methods for fatty acid analysis.

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  1. Department of Bioresources and Food Science, College of Life and Environmental Sciences, Konkuk University, Seoul, 05029, Korea

    Chang-Soo Kim, Si-Kyung Lee & Young Soo Keum

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  1. Chang-Soo Kim
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  2. Si-Kyung Lee
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Correspondence to Young Soo Keum.

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Kim, CS., Lee, SK. & Keum, Y.S. Comparative studies of immobilized lipase- and acid-catalyzed fatty acid methyl ester synthesis for seed lipid analysis. Food Sci Biotechnol 25, 771–776 (2016). https://doi.org/10.1007/s10068-016-0131-6

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  • DOI: https://doi.org/10.1007/s10068-016-0131-6

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