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Growth optimization of thraustochytrid strain 12B for the commercial production of docosahexaenoic acid

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Abstract

High yield of cell, lipid, and docosahexaenoic acid (DHA) from thraustochytrid strain 12B were achieved without the use of a complex medium and at low NaCl concentration which is detrimental to avoid unnecessary corrosion of steel tank equipment during cultivation. Culture medium that contained only 0.1% NaCl and 1% MgSO4 in an organic base solution containing 8% glucose, 1% yeast extract, and 1% peptone, referred here as NM medium, was found to be as good as or superior to the culture medium prepared from 50%(v/v) seawater with percentage lipid/dry cell weight (DCW) of 66.4%(w/w) and DHA yield up to 43.95 mg/g DCW for the thraustochytrid strain 12B. The NM medium was also applicable to the prominently high DHA-accumulating Schizochytrium limacinum SR21, and therefore this medium could probably be used for other thraustochytrid and other types of microbial strains as well.

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Authors and Affiliations

  1. Environmental Molecular Biology and Ecology, Division of Biosphere Science, Graduate School of Environmental Science, Hokkaido University, Kita-ku, Sapporo, 060-0810, Japan

    Ahmad Iskandar Bin Haji Mohd Taha & Hidetoshi Okuyama

  2. Bizen Chemical Company Ltd. Akaiwa, Okayama, 709-0716, Japan

    Takashi Kimoto & Teruyuki Kanada

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  1. Ahmad Iskandar Bin Haji Mohd Taha
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  2. Takashi Kimoto
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  3. Teruyuki Kanada
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  4. Hidetoshi Okuyama
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Correspondence to Hidetoshi Okuyama.

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Taha, A.I.B.H.M., Kimoto, T., Kanada, T. et al. Growth optimization of thraustochytrid strain 12B for the commercial production of docosahexaenoic acid. Food Sci Biotechnol 22 (Suppl 1), 53–58 (2013). https://doi.org/10.1007/s10068-013-0048-2

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