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Improvement of culture conditions for cell biomass and fatty acid production by marine thraustochytrid F24-2

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Abstract

Identification of fermentation parameters affecting biomass and total fatty acid (TFA) production by thraustochytrid F24-2 was conducted using a Plackett-Burman experimental design. Factors influencing biomass accumulation were initial pH, sea salts, and glucose concentration. Additionally, temperature, initial pH, soy peptone, glucose, and sea salt concentration affected TFA production. Docosapentaenoic acid (n-6 DPA), palmitic acid (C16:0), and docosahexaenoic acid (DHA) were the predominant fatty acids produced. The best biomass (10.71 ± 0.04 g L−1), TFA (2.11 ± 0.07 g L−1), DHA (0.92 ± 0.04 g L−1), and C16:0 (0.65 ± 0.02 g L−1) concentrations were obtained at 25 °C with a medium adjusted to pH 7 and containing (per liter): 20 g glucose, 20 g soy peptone, 18 g sea salts, 0.2 g ammonium sulfate, 3 μg vitamin B12, 3 μg biotin, and 0.6 mg thiamine hydrochloride. Under these conditions, DHA production increased 18.5% in comparison to a complex medium previously used to grow thraustochytrid Thraustochytrium aureum ATCC 34304.

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Acknowledgements

The authors are grateful to Dr. A. J. Windust, and staff at Mara, for their support. This work was supported by the grant of postdoctoral residencies abroad (No. 232236 and No. 259588) from The Mexican National Council for Science and Technology (CONACYT) and the Natural Sciences and Engineering Research Council (NSERC) of Canada.

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Authors and Affiliations

  1. Department of Process Engineering and Applied Science, Dalhousie University, Halifax, NS, B3H 4R2, Canada

    Violeta Ugalde, Roberto E. Armenta, Azadeh Kermanshahi-pour & Marianne S. Brooks

  2. Mara Renewables Corporation, 101A Research Drive, Dartmouth, NS, B2Y 4T6, Canada

    Violeta Ugalde, Roberto E. Armenta, Zhiyong Sun & Kevin T. Berryman

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  1. Violeta Ugalde
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  2. Roberto E. Armenta
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  6. Marianne S. Brooks
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Correspondence to Marianne S. Brooks.

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Ugalde, V., Armenta, R.E., Kermanshahi-pour, A. et al. Improvement of culture conditions for cell biomass and fatty acid production by marine thraustochytrid F24-2. J Appl Phycol 30, 329–339 (2018). https://doi.org/10.1007/s10811-017-1274-4

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  • DOI: https://doi.org/10.1007/s10811-017-1274-4

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