Abstract
For the purpose of understanding the molecular processes triggered during callus formation in macaw palm, the expression of seven genes potentially involved in this process, identified in previous studies and from the literature, was investigated by RT-qPCR. In addition, in situ hybridization of the SERK gene was performed. Leaf tissues from adult plants from two macaw palm accession were inoculated in a medium combined with Picloram at a concentration of 450 μM to induce callus. The expression analysis was performed from leaf samples from two accessions of different origins (Municipalities of Tiros, MG, and Buriti Vermelho, DF, Brazil), which are characterized as non-responsive (NR) and responsive (R), respectively. The material was collected before callus induction (0 DAI, initial day) and 120 days after callus induction (120 DAI). Genes related to development (SERK, OASA, EF1, ANN1) and stress (LEA, CAT2, and MDAR5) were evaluated. The results obtained showed that all the genes involved with the development had their expressions downregulated at 0 DAI when the accession R was compared with the accession NR. On the other hand, it was possible to observe that these genes were upregulated at 120 DAI. The LEA stress gene showed a tendency to increase expression in the NR accession, while the R accession showed decreased expression and the CAT2 and MDAR5 genes showed upregulation in both accessions. In situ hybridization showed SERK transcripts in the vascular bundles, indicating the expression of SERK in this region, in addition to its expression in calluses. The results obtained in this study support our hypothesis that the regulation of genes involved in the control of oxidative stress and development is crucial for the formation of calluses in macaw palm.
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The datasets generated during and/or analyzed during the current study are available from the corresponding author on reasonable request.
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Acknowledgements
The authors thank Dr. Leo Duc Haa Carson Schwartzhaupt da Conceição from Embrapa Cerrados, Brasília, DF, Brazil, for making plant material available for carrying out the experiments.
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This research was supported by Financiadora de Estudos e Projetos (Finep 01.13.0315.00), Conselho Nacional de Desenvolvimento Científco e Tecnológico (CNPq Grant 308731/2019–0), and Fundação de Apoio à Pesquisa do Distrito Federal (FAP/DF Grant 0193.001759/2017).
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J. E. S.-P. and A. M. conceived and designed this study. F. S. M., D. G. R., S. S. C., L. L. F., and A. C. M. M. G. conducted analysis. D. M. A. D. and L. H. M. contributed the analytical methods. F. S. M., J. E. S.-P., A. M., and D. M. A. D wrote the manuscript. J. E. S-P. edited the manuscript. All authors have read and agreed to the published version of the manuscript.
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Meira, F.S., Ribeiro, D.G., de Campos, S.S. et al. Differential expression of genes potentially related to the callogenesis and in situ hybridization of SERK gene in macaw palm (Acrocomia aculeata Jacq.) Lodd. ex Mart.. Protoplasma 261, 89–101 (2024). https://doi.org/10.1007/s00709-023-01881-3
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DOI: https://doi.org/10.1007/s00709-023-01881-3