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Molecular characterization of a novel mycovirus from binucleate Rhizoctonia AG-A strain A46

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Abstract

The complete genome sequence of a positive-sense single-stranded RNA (+ ssRNA) virus, Rhizoctonia beny-like virus 1 (RBLV1), isolated from binucleate Rhizoctonia AG-A strain A46, was determined. The RBLV1 genome is 10,280 nt in length and contains a short stretch of adenines at the 3′ terminus. It contains a single open reading frame (ORF) encoding a 376.30-kDa protein with viral helicase and RNA-dependent RNA polymerase (RdRp) motifs. The encoded protein exhibited the highest sequence similarity to Rhizoctonia cerealis beny-like virus 0928-1 (RcBeLV 0928-1, 45.25%), with a sequence coverage of 63%. Phylogenetic analysis based on ORF protein sequences revealed that RBLV1 is a novel unclassified mycovirus.

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Acknowledgements

This research was supported by the National Natural Scientific Foundation of China (32260036, 42067009, 32001684), the Scientific Research Fund of the Education Department of Yunnan Province (2022Y696), and the Yunnan Provincial Science and Technology Department Surface Project (202201AT070020).

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Authors and Affiliations

  1. College of Agriculture and Life Sciences, Kunming University, Kunming, Yunnan, 650214, China

    Rui Shi, Zebin Chen, Wurong Chen, Shuhui Yu, He Liu, Yuchuan Li, Lili Dai & Yanqiong Li

  2. Yunnan Academy of Tobacco Agricultural Sciences, Kunming, Yunnan, 650021, China

    Rui Shi & Xiaohan Mo

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  1. Rui Shi
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Correspondence to Yanqiong Li.

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Shi, R., Mo, X., Chen, Z. et al. Molecular characterization of a novel mycovirus from binucleate Rhizoctonia AG-A strain A46. Arch Virol 169, 31 (2024). https://doi.org/10.1007/s00705-024-05963-2

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