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Complete genome sequence of a novel fusarivirus from Rhizoctonia solani AG-3 PT strain 3P-2-2

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Abstract

Here, we describe a novel mycovirus, tentatively designated as “Rhizoctonia solani fusarivirus 6” (RsFV6), which was discovered in Rhizoctonia solani AG-3 PT strain 3P-2-2. The virus has a single-stranded positive-sense RNA (+ssRNA) genome of 6141 nucleotides containing two open reading frames (ORFs) and a poly(A) tail. ORF1 encodes a large polypeptide of 1,862 amino acids (aa) with conserved RNA-dependent RNA polymerase (RdRp) and helicase (Hel) domains. ORF2 encodes a putative 167-aa protein of unknown function. BLASTp searches revealed that the ORF1-encoded polypeptide showed the highest sequence similarity (70.67% identity) to that of Rhizoctonia solani fusarivirus 3 (RsFV3), which was isolated from Rhizoctonia solani AG-2-2LP. Multiple sequence alignments and phylogenetic analysis based on RdRp and Hel sequences indicated that RsFV6 could be a novel member of the genus Alphafusarivirus family Fusariviridae.

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All data obtained from the experiments conducted by the authors.

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Funding

The authors have not disclosed any funding. This work was supported by the National Natural Science Foundation of China (No. 31960525, No. 31160352, and No. 31360423) and the National Natural Scientific Foundation of Yunnan (No. 202207AA110005).

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Authors and Affiliations

  1. State Key Laboratory for Protection and Utilization of Bio-Resources in Yunnan, Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    Aili Sun, Li Luo, Zifang Zhao, Lijuan Kang, Chengyun Li & Genhua Yang

  2. Technology Department, Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    Wenhan Dong

Authors
  1. Aili Sun
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  2. Li Luo
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  3. Zifang Zhao
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  4. Lijuan Kang
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  5. Chengyun Li
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  6. Wenhan Dong
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  7. Genhua Yang
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Correspondence to Wenhan Dong or Genhua Yang.

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Handling Editor: Ioly Kotta-Loizou.

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Sun, A., Luo, L., Zhao, Z. et al. Complete genome sequence of a novel fusarivirus from Rhizoctonia solani AG-3 PT strain 3P-2-2. Arch Virol 168, 75 (2023). https://doi.org/10.1007/s00705-022-05673-7

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  • DOI: https://doi.org/10.1007/s00705-022-05673-7

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