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Identification of AG-V, a new anastomosis group of binucleate Rhizoctonia spp. from taro and ginger in Yunnan province

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Abstract

Binucleate Rhizoctonia (BNR) spp. isolates were collected from taro (Colocasia esculenta (L.) Schott) and ginger (Zingiber officinale (Willd.) Roscoe) (Yunnanxiaojiang cv.) in Yunnan province. These Yunnan (YN) isolates did not anastomose with any of the tester isolates of the known AGs of binucleate Rhizoctonia spp. The growth of YN cultures on PDA was appressed, mealy and matlike after 4 days of incubation, then turned white brown, producing brown to dark brown, irregularly shaped sclerotia were embedded in the PDA medium after 14 days. All attempts to induce basidiospore production were unsuccessful, but the length and sequence of the internal transcribed spacer (ITS1 + 5.8S rDNA + ITS2) regions of 5.8S rDNA from the YN isolates were identical in length and sequence to isolates of all the other AGs of binucleate Rhizoctonia /Ceratobasidium spp. The sequences of 5.8S rDNA-ITS from the YN isolates were unique among AGs of BNR. The YN isolates had sequence similarities of 94% with isolates of AG Fb and P, 93% with AG E, 91% with AG R, 79–94% with AG S, and 74–87% with AG A, Ba, Bb, Bo, C, DI, DII, DIII, Fa, G, H, I, K, L, O, and Q. Four isolates of AG YN caused minor virulence (lesions ≦1mm2) to ginger or taro in growth chamber studies. It was concluded that the YN isolates belong to a new anastomosis group AG-V of the Ceratobasidium spp..

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Acknowledgements

This research was supported by the National Natural Scientific fund (30660006, 31160352) and Program for Innovative Research Team (in Science and Technology) at the University of Yunnan Province.

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Authors and Affiliations

  1. Key Laboratory of Agro-Biodiversity and Pest Management of Education Ministry of China, Yunnan Agricultural University, Kunming, 650201, China

    Wenhan Dong, Xiaoguang Li, Genhua Yang & Chengyun Li

  2. Agriculture College, Kunming University, Kunming, 650214, China

    Yanqiong Li

  3. Yunnan Academy of Agricultural Sciences, Institute of Tropical and Subtropical Cash-Crops Research, North Lancheng Road in Longyang County, Baoshan City, Yunnan Province, 678000, China

    Chunfang Duan

  4. Agriculture, Forestry and Fisheries Technical Information Society, Nihonbashi-Kabutocho, Chuo-Ku, Tokyo, 103-00264, Japan

    Shigeo Naito

  5. Agriculture and Agri-Food Canada, Cereal Research Centre-Morden, Unit 101, Route 100, Morden, MB, R6M 1Y5, Canada

    Robert L. Conner

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  1. Wenhan Dong
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  2. Yanqiong Li
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  3. Chunfang Duan
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  4. Xiaoguang Li
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  5. Shigeo Naito
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  6. Robert L. Conner
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  7. Genhua Yang
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  8. Chengyun Li
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Corresponding authors

Correspondence to Genhua Yang or Chengyun Li.

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Wenhan Dong and Yanqiong Li contributed equally to this work.

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Dong, W., Li, Y., Duan, C. et al. Identification of AG-V, a new anastomosis group of binucleate Rhizoctonia spp. from taro and ginger in Yunnan province. Eur J Plant Pathol 148, 895–906 (2017). https://doi.org/10.1007/s10658-016-1144-y

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  • DOI: https://doi.org/10.1007/s10658-016-1144-y

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