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Silver nanoparticles as a viricidal agent to inhibit plant-infecting viruses and disrupt their acquisition and transmission by their aphid vector

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Abstract

Silver nanoparticles (AgNPs) are a potentially effective tool for preventing viral plant diseases. This study was carried out to evaluate the effectiveness of AgNPs for managing bean yellow mosaic virus (BYMV) disease in faba bean plants from the plant-virus-vector interaction side. AgNPs were evaluated as foliar protective and curative agents. In addition, the effect of AgNPs on virus acquisition and transmission by its vector aphid was investigated. The results indicated that AgNPs exhibited curative viricidal activity and were able to inactivate BYMV when applied 48 hours after virus inoculation. The occurrence of disease was prevented using an AgNP concentration as low as 100 mg L-1, whereas virus infection was completely inhibited when plants were preventatively treated with AgNPs at a concentration of to 200 mg L-1 24 h before virus inoculation. AgNPs proved to be highly bio-reactive, binding to viral particles and suppressing their replication and accumulation within plant tissues. Moreover, AgNPs, at all concentrations tested, were found to upregulate the pathogenesis-related gene PR-1 and induce the production of defense-related oxidizing enzymes in treated plants. Exposure of aphids to AgNPs-treated plants before virus acquisition reduced BYMV acquisition and transmission efficiency by 40.65 to 100% at 24 h post-application, depending on the AgNP dosage. At 10 days after treatment, virus acquisition was reduced by 36.82% and 79.64% upon exposure to AgNPs at a concentration of 250 and 300 mg L-1, respectively. These results suggest that AgNPs have curative viricidal activity due to targeting the virus coat protein and affecting virus-vector interactions. Accordingly, AgNPs may contribute to alleviating the natural disease and virus transmission under field conditions. This is the first report on the activity of nanomaterials against plant virus acquisition and transmission by insects.

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Acknowledgements

The authors would like to thank the Central Laboratory of Nanotechnology & Advanced Materials, Agricultural Research Center (ARC), Giza, Egypt, for providing the laboratory facilities for the characterization of synthesized silver nanoparticles.

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Authors and Affiliations

  1. Virus and Phytoplasma Research Department, Plant Pathology Research Institute, Agricultural Research Center (ARC), Giza, 12619, Egypt

    Ahmed Y. El Gamal & Tarek El Sayed

  2. Plant Pathology Department, Faculty of Agriculture, Zagazig University, Zagazig, Egypt

    Mohamed R. Tohamy, Mohamed I. Abou-Zaid & Mahmoud M. Atia

  3. Nanotechnology and Advanced Materials Central Lab., Agricultural Research Center (ARC), Giza, Egypt

    Khaled Y. Farroh

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  1. Ahmed Y. El Gamal
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El Gamal, A.Y., Tohamy, M.R., Abou-Zaid, M.I. et al. Silver nanoparticles as a viricidal agent to inhibit plant-infecting viruses and disrupt their acquisition and transmission by their aphid vector. Arch Virol 167, 85–97 (2022). https://doi.org/10.1007/s00705-021-05280-y

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