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Complete nucleotide sequence of a novel mycovirus infecting Rhizoctonia fumigata AG-Ba isolate C-314 Baishi

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Abstract

The complete nucleotide sequence of a novel mycovirus, designated as "Rhizoctonia fumigata bipartite virus 1" (RfBV1), from Rhizoctonia fumigata AG-Ba isolate C-314 Baishi was determined. The genome of RfBV1 is composed of two double-stranded RNAs (dsRNA). dsRNA-1 (2311 bp) contains one open reading frame (ORF), which codes for the putative RNA-dependent RNA polymerase (RdRp) of the virus. dsRNA-2 (1690 bp) contains one ORF, which encodes a putative protein whose function is unknown. Phylogenetic analysis indicated that the RdRp of RfBV1 clustered with several unassigned bipartite viruses belonging to the CThTV-like viruses group, but not the family Amalgaviridae or Partitiviridae. Our study suggests that RfBV1 is a novel mycovirus related to the CThTV-like viruses.

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Acknowledgements

The authors would like to thank Dr. A. Ogashi from Hokkaido University, Japan, for kindly providing the Rhizoctonia fumigata AG-Ba isolate C-314 Baishi used in this study. This research was supported by Kunming University (YJL16006), Yunnan Provincial Department of Science and Technology (2018FH001-032, 2018FH001-037, 2018FD080), the National Natural Science Foundation of China (31960525), and Ningbo University (KF20190107).

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Authors and Affiliations

  1. College of Agriculture and Life Sciences, Kunming University, Kunming, 650214, Yunnan, China

    Yanqiong Li, Wurong Chen & Zhen Ren

  2. College of Plant Protection, Yunnan Agricultural University, Kunming, 650201, Yunnan, China

    Yanqiong Li, Ping Xu, Lifang Zhang & Genhua Yang

  3. College of Bioresources and Food Engineering, Qujing Normal University, Qujing, 655011, Yunnan, China

    Lifang Zhang

  4. Yunnan Academy of Tobacco Agricultural Sciences, Kunming, 650021, Yunnan, China

    Yanqiong Li, Ping Xu, Lifang Zhang & Xiaohan Mo

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  1. Yanqiong Li
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  2. Ping Xu
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  6. Genhua Yang
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Correspondence to Genhua Yang or Xiaohan Mo.

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Handling Editor: Ioly Kotta-Loizou.

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The nucleotide sequence data reported here are available in the DDBJ/EMBL/GenBank databases under the accession numbers KP209316 and KP209317.

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Li, Y., Xu, P., Zhang, L. et al. Complete nucleotide sequence of a novel mycovirus infecting Rhizoctonia fumigata AG-Ba isolate C-314 Baishi. Arch Virol 167, 959–963 (2022). https://doi.org/10.1007/s00705-021-05269-7

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  • DOI: https://doi.org/10.1007/s00705-021-05269-7

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