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Full genome sequence of a new mitovirus from the phytopathogenic fungus Rhizoctonia solani

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Abstract

A double-stranded RNA (dsRNA) segment was identified in Rhizoctonia solani anastomosis group (AG)-2-2IIIB, the primary causal agent of Rhizoctonia crown and root rot of sugar beet. The dsRNA segment represented the genome replication intermediate of a new mitovirus that was tentatively designated as "Rhizoctonia solani mitovirus 39" (RsMV-39). The complete sequence of the dsRNA was 2805 bp in length with 61.9% A+U content. Using either the fungal mitochondrial or universal genetic code, a protein of 840 amino acids containing an RNA-dependent RNA polymerase (RdRp) domain was predicted with a molecular mass of 94.46 kDa. BLASTp analysis revealed that the RdRp domain of RsMV-39 had 43.55% to 72.96% sequence identity to viruses in the genus Mitovirus, and was the most similar (72.96% identical) to that of Ceratobasidium mitovirus A (CbMV-A). Phylogenetic analysis based on RdRp domains clearly showed that RsMV-39 is a member of a distinct species in the genus Mitovirus of the family Mitoviridae. This is the first full genome sequence of a mycovirus associated with R. solani AG-2-2IIIB.

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Funding

This work was financially supported by the Earmarked Fund for China Agriculture Research System (CARS-170304) and the National Training Program of Innovation and Entrepreneurship for Undergraduates (201910019031).

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Authors and Affiliations

  1. College of Plant Protection, China Agricultural University, Haidian District, Beijing, 100193, People’s Republic of China

    Siwei Li, Yuting Li, Chenghui Hu, Chenggui Han, Tao Zhou, Can Zhao & Xuehong Wu

  2. College of Horticulture, China Agricultural University, Haidian District, Beijing, 100193, People’s Republic of China

    Can Zhao

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  1. Siwei Li
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  2. Yuting Li
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  3. Chenghui Hu
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  4. Chenggui Han
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  5. Tao Zhou
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  6. Can Zhao
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  7. Xuehong Wu
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Correspondence to Can Zhao or Xuehong Wu.

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Li, S., Li, Y., Hu, C. et al. Full genome sequence of a new mitovirus from the phytopathogenic fungus Rhizoctonia solani. Arch Virol 165, 1719–1723 (2020). https://doi.org/10.1007/s00705-020-04664-w

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  • DOI: https://doi.org/10.1007/s00705-020-04664-w

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