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Molecular characterization of a novel partitivirus and a fusarivirus coinfecting the fungus Nigrospora sphaerica

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Abstract

In this report, we describe the molecular characterization of two novel mycoviruses coinfecting the plant pathogenic fungus Nigrospora sphaerica, which were designated "Nigrospora sphaerica fusarivirus 1" (NsFV1) and "Nigrospora sphaerica partitivirus 1" (NsPV1). NsFV1 has an undivided genome measuring 6,147 nt, excluding the polyA tail, and was predicted to contain two nonoverlapping open reading frames (ORF1 and 2). The larger ORF1 encodes a polyprotein containing a conserved RNA-dependent RNA polymerase (RdRp) and a helicase domain that has functions related to RNA replication, and the smaller ORF2 encodes a putative protein with an unknown function. NsPV1 consists of two genome segments, which measure 1,796 bp and 1,455 bp in length. Each of the two dsRNAs has a single ORF, and they are predicted to encode proteins with homology to viral RdRps and coat proteins of members of the family Partitiviridae. Phylogenetic analysis indicated that NsFV1 is a member of the recently proposed family "Fusariviridae", while NsPV1 was determined to belong to the genus Gammapartitivirus in the family Partitiviridae. To the best of our knowledge, this report is the first to describe mycoviruses infecting N. sphaerica.

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References

  1. Pearson MN, Beever RE, Boine B, Arthur K (2009) Mycoviruses of filamentous fungi and their relevance to plant pathology. Mol Plant Pathol 10:115–128

    Article  CAS  Google Scholar 

  2. Xie J, Jiang D (2014) New insights into mycoviruses and exploration for the biological control of crop fungal diseases. Annu Rev Phytopathol 52:45–68

    Article  CAS  Google Scholar 

  3. Ghabrial SA, Castón JR, Jiang D, Nibert ML, Suzuki N (2015) 50-plus years of fungal viruses. Virology 479:356–368

    Article  Google Scholar 

  4. Hillman BI, Annisa A, Suzuki N (2018) Viruses of plant-interacting fungi. In: Advances in Virus Research (Vol 100, pp 99–116). Academic Press, Hoboken

  5. Marzano SYL, Nelson BD, Ajayi-Oyetunde O, Bradley CA, Hughes TJ, Hartman GL, Eastburn DM, Domier LL (2016) Identification of diverse mycoviruses through metatranscriptomics characterization of the viromes of five major fungal plant pathogens. J Virol 90:6846–6863

    Article  CAS  Google Scholar 

  6. Nuss DL (2005) Hypovirulence: mycoviruses at the fungal-plant interface. Nat Rev Microbiol 3:632–642

    Article  CAS  Google Scholar 

  7. Roossinck MJ (2015) Move over, bacteria! Viruses make their mark as mutualistic microbial symbionts. J Virol 89:6532–6535

    Article  CAS  Google Scholar 

  8. Kotta-Loizou I, Coutts RH (2017) Studies on the virome of the entomopathogenic fungus Beauveria bassiana reveal novel dsRNA elements and mild hypervirulence. PLoS Pathog 13:e1006183

    Article  Google Scholar 

  9. Márquez LM, Redman RS, Rodriguez RJ, Roossinck MJ (2007) A virus in a fungus in a plant: three-way symbiosis required for thermal tolerance. Science 315:513–515

    Article  Google Scholar 

  10. Nerva L, Silvestri A, Ciuffo M, Palmano S, Varese GC, Turina M (2017) Transmission of Penicillium aurantiogriseum partiti-like virus 1 to a new fungal host (Cryphonectria parasitica) confers higher resistance to salinity and reveals adaptive genomic changes. Environ Microbiol 19:4480–4492

    Article  CAS  Google Scholar 

  11. Nerva L, Chitarra W, Siciliano I, Gaiotti F, Ciuffo M, Forgia M, Turina M (2019) Mycoviruses mediate mycotoxin regulation in Aspergillus ochraceus. Environ Microbiol 21:1957–1968

    Article  CAS  Google Scholar 

  12. Chun J, Yang HE, Kim DH (2018) Identification of a novel partitivirus of Trichoderma harzianum NFCF319 and evidence for the related antifungal activity. Front Plant Sci 9:1699

    Article  Google Scholar 

  13. Roossinck MJ (2019) Evolutionary and ecological links between plant and fungal viruses. New Phytol 221:86–92

    Article  Google Scholar 

  14. Yu X, Li B, Fu Y (2010) A geminivirus-related DNA mycovirus that confers hypovirulence to a plant pathogenic fungus. Proc Natl Acad Sci USA 107:8387–8392

    Article  CAS  Google Scholar 

  15. Li P, Wang S, Zhang L, Qiu D, Zhou X, Guo L (2020) A tripartite ssDNA mycovirus from a plant pathogenic fungus is infectious as cloned DNA and purified virions. Sci Adv 6:eaay9634

    Article  CAS  Google Scholar 

  16. Liu LJ, Xie JT, Cheng JS, Fu YP, Li GQ, Yi XH, Jiang DH (2014) Fungal negative-stranded RNA virus that is related to bornaviruses and nyaviruses. Proc Natl Acad Sci USA 111:12205–12210

    Article  CAS  Google Scholar 

  17. Vainio EJ, Chiba S, Ghabrial SA, Maiss E, Roossinck M, Sabanadzovic S, Suzuki N, Xie J, Nibert M, ICTV Report Consortium (2018) ICTV virus taxonomy profile: Partitiviridae. J Gen Virol 99:17–18

    Article  CAS  Google Scholar 

  18. Kwon SJ, Lim WS, Park SH, Park MR, Kim KH (2007) Molecular characterization of a dsRNA mycovirus, Fusarium graminearum virus-DK21, which is phylogenetically related to hypoviruses but has a genome organization and gene expression strategy resembling those of plant potex-like viruses. Mol Cells 23:304

    CAS  PubMed  Google Scholar 

  19. Zhang R, Liu S, Chiba S, Kondo H, Kanematsu S, Suzuki N (2014) A novel single-stranded RNA virus isolated from a phytopathogenic filamentous fungus, Rosellinia necatrix, with similarity to hypo-like viruses. Front Microbiol 5:360

    PubMed  PubMed Central  Google Scholar 

  20. Zhong J, Shang HH, Zhu CX, Zhu JZ, Hu Y, Gao BD (2016) Characterization of a novel single-stranded RNA virus, closely related to fusariviruses, infecting the plant pathogenic fungus Alternaria brassicicola. Virus Res 217:1–7

    Article  CAS  Google Scholar 

  21. Metwaly AM, Kadry HA, Atef A, Mohammad AEI, Ma G, Cutler SJ, Ross SA (2014) Nigrosphaerin A a new isochromene derivative from the endophytic fungus Nigrospora sphaerica. Phytochem Lett 7:1–5

    Article  CAS  Google Scholar 

  22. Wu Z, Xie Z, Wu M, Li X, Li W, Ding W, She ZG, Li C (2018) New antimicrobial cyclopentenones from Nigrospora sphaerica ZMT05, a fungus derived from Oxya chinensis Thunber. J Agr Food Chem 66:5368–5372

    Article  CAS  Google Scholar 

  23. Farr DF, Rossman AY (2020) Fungal Databases, US National Fungus Collections, ARS, USDA. https://nt.ars-grin.gov/fungaldatabases/. Accessed 20 Jul 2020

  24. Prasher IB, Dhanda RK (2017) GC-MS analysis of secondary metabolites of endophytic Nigrospora sphaerica isolated from Parthenium hysterophorus. Int J Pharm Sci Rev Res 44:217–223

    CAS  Google Scholar 

  25. Zhou Q, Zhong J, Hu Y, Gao BD (2016) A novel nonsegmented double-stranded RNA mycovirus identified in the phytopathogenic fungus Nigrospora oryzae shows similarity to partitivirus-like viruses. Arch Virol 161:229–232

    Article  CAS  Google Scholar 

  26. Zhong J, Zhao SQ, Li GF, Pang XD, Deng XJ, Zhu HJ, Gao BD, Zhou Q (2016) A novel fusarivirus isolated from the phytopathogenic fungus Nigrospora oryzae. Virus Genes 52:891–895

    Article  CAS  Google Scholar 

  27. Zhong J, Zhou Q, Hu Y, Zhu HJ, Gao BD (2016) Molecular identification of a novel victorivirus from the phytopathogenic fungus Nigrospora oryzae. Virus Genes 52:156–159

    Article  CAS  Google Scholar 

  28. Liu H, Liu R, Li CX, Wang H, Zhu HJ, Gao BD, Zhou Q, Zhong J (2019) A victorivirus and two novel mitoviruses co-infected the plant pathogen Nigrospora oryzae. Viruses 11:83

    Article  CAS  Google Scholar 

  29. Morris TJ, Dodds JA (1979) Isolation and analysis of double stranded RNA from virus-infected plant and fungal tissue. Phytopathology 69:854–858

    Article  CAS  Google Scholar 

  30. Zhong J, Lei XH, Zhu JZ, Song G, Zhang YD, Chen Y, Gao BD (2014) Detection and sequence analysis of two novel co-infecting double-strand RNA mycoviruses in Ustilaginoidea virens. Arch Virol 159:3063–3070

    Article  CAS  Google Scholar 

  31. Thompson JD, Gibson TJ, Plewniak F, Jeanmougin F, Higgins DG (1997) The CLUSTAL_X windows interface: flexible strategies for multiple sequence alignment aided by quality analysis tools. Nucleic Acids Res 25:4876–4882

    Article  CAS  Google Scholar 

  32. Tamura K, Stecher G, Peterson D, Filipski A, Kumar S (2013) MEGA6: molecular evolutionary genetics analysis version 6.0. Mol Biol Evol 30:2725–2729

    Article  CAS  Google Scholar 

  33. Hrabáková L, Grum-Grzhimaylo AA, Koloniuk I, Debets AJ, Sarkisova T, Petrzik K (2017) The alkalophilic fungus Sodiomyces alkalinus hosts beta-and gammapartitiviruses together with a new fusarivirus. PLoS ONE 12:e0187799

    Article  Google Scholar 

  34. Buck KW (1996) Comparison of the replication of positive stranded RNA viruses of plants and animals. Adv Virus Res 47:159–251

    Article  CAS  Google Scholar 

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Acknowledgements

This research was supported by the China Postdoctoral Science Foundation (grant no. 2018M641562).

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Author notes

  1. Jie Zhong and Ze Zhong Yang contributed equally to this work.

Authors and Affiliations

  1. Department of Plant Protection, Institute of Vegetables and Flowers, Chinese Academy of Agricultural Sciences, Beijing, 100081, China

    Jie Zhong, Ze Zhong Yang, Xin Yang, Zhao Jiang Guo, Wen Xie & You Jun Zhang

  2. Hunan Provincial Key Laboratory for Biology and Control of Plant Diseases and Insect Pests, Hunan Agricultural University, Nongda Road 1, Furong District, Changsha City, Hunan Province, 410128, People’s Republic of China

    Jie Zhong

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  1. Jie Zhong
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Correspondence to You Jun Zhang.

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Zhong, J., Yang, Z.Z., Yang, X. et al. Molecular characterization of a novel partitivirus and a fusarivirus coinfecting the fungus Nigrospora sphaerica. Arch Virol 166, 2325–2331 (2021). https://doi.org/10.1007/s00705-021-05095-x

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