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A novel mycovirus infecting Aspergillus nidulans that is closely related to viruses in a new genus of the family Partitiviridae

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Abstract

The bisegmented genome of a novel double-stranded (ds) RNA mycovirus, named “Aspergillus nidulans partitivirus 1” (AnPV1), isolated from the fungus Aspergillus nidulans strain HJ5-47, was sequenced and analyzed. AnPV1 contains two segments, AnPV1-1 and AnPV1-2. AnPV1-1 has 1837 bp with an open reading frame (ORF) that potentially encodes a putative RNA-dependent RNA polymerase (RdRp) of 572 amino acids (aa). AnPV1-2 has 1583 bp with an ORF encoding a putative capsid protein (CP) of 488 aa. Phylogenetic analyses indicated that AnPV1 and related viruses clustered in a group that could represent a new unclassified genus in the family Partitiviridae.

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Funding

This study was funded by the National Natural Science Foundation of China (31660012 and 32000017), the Science and Technology Foundation of Guizhou Province [(2019)1277], the Regional Common Diseases and Adult Stem Cell Transformation Research and Innovation Platform of Guizhou Provincial Department of Science and Technology [(2019)4008], the Joint Foundation of Collaboration Project between Scientific and Technological Bureau of Guizhou Province and Universities of Guizhou Province [LH(2016)7348], the Joint Foundation of Collaboration Project between Scientific and Technological Bureau of Guiyang City and Guizhou Medical University [(20161001)032], and the Youth Science and Technology Talent Development Project from Guizhou Provincial Department of Education [KY(2016)146].

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Author notes

  1. Yinhui Jiang and Bi Yang are contributed equally to this work.

Authors and Affiliations

  1. Key Laboratory of Endemic and Ethnic Diseases, Ministry of Education, Guizhou Medical University, Guiyang, 550004, China

    Yinhui Jiang, Bi Yang, Qinrong Wang, Qifang Zhang & Wenfeng Yu

  2. Key Laboratory of Medical Molecular Biology, Guizhou Medical University, Guiyang, 550004, Guizhou Province, China

    Yinhui Jiang, Bi Yang, Qinrong Wang, Qifang Zhang & Wenfeng Yu

  3. Department of Biology, School of Basic Medical Sciences, Guizhou Medical University, Guiyang, 550004, Guizhou Province, China

    Jiayu Liu

  4. School of Environmental Sciences, University of Guelph, Guelph, ON, N1G 2W1, Canada

    Tom Hsiang

Authors
  1. Yinhui Jiang
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  2. Bi Yang
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  3. Jiayu Liu
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  4. Qinrong Wang
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  6. Wenfeng Yu
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  7. Tom Hsiang
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Correspondence to Yinhui Jiang.

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Handling Editor: Ioly Kotta-Loizou.

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F

ig. S1 Phylogenetic analysis based on CP sequences of AnPV1 and other members of the family Partitiviridae. The maximum-likelihood tree was constructed in MEGA 6.06 with 1000 bootstrap replicates. GenBank accession numbers are shown before the virus names in the tree. Helminthosporium victoriae virus 190S, a member of the genus Totivirus in the family Totiviridae, was used to root the tree. The arrow indicates the position of AnPV1. (JPG 2692 KB)

F

ig. S2 Agarose gel electrophoresis of the cDNA of AnPV1-1 and AnPV1-2. (JPG 303 KB)

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Jiang, Y., Yang, B., Liu, J. et al. A novel mycovirus infecting Aspergillus nidulans that is closely related to viruses in a new genus of the family Partitiviridae. Arch Virol 166, 659–664 (2021). https://doi.org/10.1007/s00705-020-04930-x

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  • DOI: https://doi.org/10.1007/s00705-020-04930-x

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