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A new virus of the family Tombusviridae infecting sugarcane

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Abstract

Many viral diseases of sugarcane negatively affect yield. A sugarcane accession originating from South Africa exhibiting mosaic symptoms was processed for high-throughput sequencing. Bioinformatic analysis revealed two known sugarcane viruses and a contig of around 2,800 nucleotides resembling umbra-like viruses of the family Tombusviridae. The sequence of the viral contig was confirmed by cloning and Sanger sequencing, and the ends of the virus sequence were determined. Open reading frame analysis revealed the presence of four ORFs. Phylogenetic analysis of the complete virus sequence showed that this virus clusters with other umbra-like viruses of the family Tombusviridae.

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Availability of data and material

The full genome sequence of sugarcane umbra-like virus has been deposited in the GenBank database under accession no. MN868593.

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Funding

This work was supported by the United States Department of Agriculture, Agricultural Research Service.

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Author notes

  1. Muhammad N. Tahir

    Present address: Department of Plant Pathology, Bahauddin Zakariya University, Multan, Pakistan

  2. Muhammad N. Tahir and Stephen Bolus contributed equally to this work.

Authors and Affiliations

  1. USDA ARS, National Germplasm Resources Laboratory, Beltsville, MD, 20705, USA

    Muhammad N. Tahir, Stephen Bolus, Samuel C. Grinstead & Dimitre Mollov

  2. South African Sugarcane Research Institute, Mount Edgecombe, KwaZulu-Natal, 4300, South Africa

    Sharon A. McFarlane

Authors
  1. Muhammad N. Tahir
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  2. Stephen Bolus
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  3. Samuel C. Grinstead
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  4. Sharon A. McFarlane
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  5. Dimitre Mollov
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Contributions

Conceptualization: DM, MNT. Methodology: MNT, SCG. Formal analysis and investigation: MNT, SB, DM. Writing—original draft preparation: SB, DM, MNT, SM. Writing—review and editing: SB, DM, MNT, SM, SCG. Funding acquisition: DM. Resources: DM, SM. Supervision: DM.

Corresponding author

Correspondence to Dimitre Mollov.

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Conflict of interest

We report no conflict of interest.

Ethics approval

All plant material was imported under USDA APHIS PPQ permits.

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Handling Editor: Massimo Turina.

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Tahir, M.N., Bolus, S., Grinstead, S.C. et al. A new virus of the family Tombusviridae infecting sugarcane. Arch Virol 166, 961–965 (2021). https://doi.org/10.1007/s00705-020-04908-9

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  • DOI: https://doi.org/10.1007/s00705-020-04908-9

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