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Detection and partial genome sequence of a new umbra-like virus of papaya discovered in Ecuador

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Abstract

Sequencing of cDNA synthesized from double-stranded RNA (dsRNA) from papaya leaves revealed the presence of a putative new virus with homology to genomes of several members of the genus Umbravirus. A wide survey showed that the virus was present in two out of three papaya producing provinces in Ecuador included in this study. The virus was found in up to 31 % of symptomatic trees co-infected with Papaya ringspot virus (PRSV) compared to 2 % in symptomless PRSV-free plants. Mechanical and aphid inoculations failed to transmit the virus under controlled conditions. The virus was not transmitted through seeds. The presence of a luteovirus, commonly associated with umbraviruses, was not confirmed in plants infected with the new virus using universal luteovirus primers.

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Acknowledgments

This work was partially funded by the International Foundation for Science under grant agreement No. C/5433-1. Additional funding was provided by the Ecuadorean Science and Technology Secretariat (SENESCYT), through the National Emblematic Program PROMETEO. The authors thank all the growers especially Jorge Cueva, for granting us access to commercial fields and providing us assistance with sample collection.

Compliance with Ethical Standards

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Funding

This study was partially funded by the International Foundation for Science under grant agreement No. C/5433-1. Additional funding was provided by the Ecuadorean Science and Technology Secretariat (SENESCYT), through the National Emblematic Program PROMETEO.

Conflict of Interest

The authors declare that they have no conflicts of interest.

Author information

Authors and Affiliations

  1. Centro de Investigaciones Biotecnológicas del Ecuador, Facultad de Ingeniería Mecánica y Ciencias de la Producción, Escuela Superior Politecnica del Litoral, CIBE-ESPOL, Km 30.5 Via Perimetral Campus Gustavo Galindo, apartado 09-01-5863, Guayaquil, Ecuador

    D. F. Quito-Avila, R. A. Alvarez & M. A. Ibarra

  2. Programa Prometeo, Secretaría de Educación Superior, Ciencia, Tecnología e Innovación, Quito, Ecuador

    D. F. Quito-Avila

  3. USDA-ARS, Horticultural Crops Research Unit, Corvallis, OR, 97330, USA

    R. R. Martin

Authors
  1. D. F. Quito-Avila
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  2. R. A. Alvarez
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  3. M. A. Ibarra
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  4. R. R. Martin
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Corresponding author

Correspondence to D. F. Quito-Avila.

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Quito-Avila, D.F., Alvarez, R.A., Ibarra, M.A. et al. Detection and partial genome sequence of a new umbra-like virus of papaya discovered in Ecuador. Eur J Plant Pathol 143, 199–204 (2015). https://doi.org/10.1007/s10658-015-0675-y

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