Abstract
Bermuda grass samples were examined by transmission electron microscopy and 28-30 nm spherical virus particles were observed. Total RNA from these plants was subjected to high-throughput sequencing (HTS). The nearly full genome sequence of a panicovirus was identified from one HTS scaffold. Sanger sequencing was used to confirm the HTS results and complete the genome sequence of 4404 nt. This virus was provisionally named Bermuda grass latent virus (BGLV). Its predicted open reading frames follow the typical arrangement of the genus Panicovirus. Based on sequence comparisons and phylogenetic analyses BGLV differs from other viruses and therefore taxonomically it is a new member of the genus Panicovirus, family Tombusviridae.
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All authors declare they have no conflict of interest. The research reported in this manuscript did not involve experimentation with humans or animals. Plant samples were moved within USA under USDA APHIS permit P526P-13-03719.
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Tahir, M.N., Lockhart, B., Grinstead, S. et al. Characterization and complete genome sequence of a panicovirus from Bermuda grass by high-throughput sequencing. Arch Virol 162, 1099–1102 (2017). https://doi.org/10.1007/s00705-016-3165-4
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DOI: https://doi.org/10.1007/s00705-016-3165-4