Abstract
The complete genomic sequence of a putative novel member of the family Secoviridae was determined by high-throughput sequencing of a pineapple accession obtained from the National Plant Germplasm Repository in Hilo, Hawaii. The predicted genome of the putative virus was composed of two RNA molecules of 6,128 and 4,161 nucleotides in length, excluding the poly-A tails. Each genome segment contained one large open reading frame (ORF) that shares homology and phylogenetic identity with members of the family Secoviridae. The presence of this new virus in pineapple was confirmed using RT-PCR and Sanger sequencing from six samples collected in Oahu, Hawaii. The name “pineapple secovirus A” (PSVA) is proposed for this putative new sadwavirus.
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Funding
This research was supported in part by grants from the United States Department of Agriculture National Institute of Food and Agriculture, Hatch HAW09025-H (1001478), and the United State Department of Agriculture -Agricultural Research Service (58-5320-4-012).
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Communicated by Jesús Navas-Castillo.
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Larrea-Sarmiento, A., Olmedo-Velarde, A., Green, J.C. et al. Identification and complete genomic sequence of a novel sadwavirus discovered in pineapple (Ananas comosus). Arch Virol 165, 1245–1248 (2020). https://doi.org/10.1007/s00705-020-04592-9
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DOI: https://doi.org/10.1007/s00705-020-04592-9