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Colorimetric and visual determination of uric acid based on decolorization of manganese dioxide nanosheet dispersions

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Abstract

Serum levels of uric acid (UA) play an important role in the prevention of diseases. Developing a rapid and accurate way to detect UA is still a meaningful task. Hence, positively charged manganese dioxide nanosheets (MnO2NSs) with an average latter size of 100 nm and an ultra-thin thickness of below 1 nm have been prepared. They can be well dispersed in water and form stable yellow-brown solutions. The MnO2NSs can be decomposed by UA via redox reaction, leading to a decline of a characteristic absorption peak (374 nm) and a color fading of MnO2NSs solution. On this basis, an enzyme-free colorimetric sensing system for the detection of UA has been developed. The sensing system shows many advantages, including a wide linear range of 0.10–50.0 μmol/L, a limit of quantitation (LOQ) of 0.10 μmol/L, a low limit of detection (LOD) of 0.047 μmol/L (3σ/m), and rapid response without need of strict time control. Moreover, a simple and convenient visual sensor for UA detection has also been developed by adding an appropriate amount of phthalocyanine to provide a blue background color, which helps to increase visual discrimination. Finally, the strategy has been successfully applied to detect UA in human serum and urine samples.

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Funding

This study was financially supported by the National Natural Science Foundation of China (21976029) and the Natural Science Foundation of Fujian Province (2020Y0074, 2022Y0059, 2022J0113).

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Authors and Affiliations

  1. MOE Key Laboratory for Analytical Science of Food Safety and Biology, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, College of Chemistry, Fuzhou University, Fuzhou, China

    Rongjing Hu, Tianmin Guo, Chenyi Zeng, Xiaolong Fu, Zhenyu Lin, Yongqiang Dong & Fengfu Fu

  2. Laboratory of Gynecologic Oncology, Department of Gynecology, Fujian Maternity and Child Health Hospital, College of Clinical Medicine for Obstetrics & Gynecology and Pediatrics, Fujian Medical University, Fuzhou, China

    Binhua Dong

Authors
  1. Rongjing Hu
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  2. Tianmin Guo
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  3. Chenyi Zeng
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  4. Xiaolong Fu
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  5. Binhua Dong
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  6. Zhenyu Lin
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  7. Yongqiang Dong
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  8. Fengfu Fu
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Contributions

Prof. Y. Dong and Prof. F. Fu performed the experimental design, data analysis and interpretation, and manuscript writing. R. Hu, T. Guo, C. Zeng, and X. Fu performed the experiments. Prof. Z. Lin and Dr. B. Dong co-performed data analysis and interpretation. The manuscript was written through contributions of all authors, and all authors have given approval to the final version of the manuscript.

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Correspondence to Yongqiang Dong or Fengfu Fu.

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Hu, R., Guo, T., Zeng, C. et al. Colorimetric and visual determination of uric acid based on decolorization of manganese dioxide nanosheet dispersions. Microchim Acta 190, 217 (2023). https://doi.org/10.1007/s00604-023-05767-2

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  • DOI: https://doi.org/10.1007/s00604-023-05767-2

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