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Colorimetric determination of glutathione by using a nanohybrid composed of manganese dioxide and carbon dots

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Abstract

A kind of single-layer carbon based dots (CDs) with abundant carboxyl functional groups was hybridized with manganese dioxide (MnO2). The resulting nanohybrid is stable and can be well dispersed in water. MnO2 is capable of oxidizing the substrate 3,3′5,5′-tetramethylbenzidine (TMB) to form a blue product whose absorption (peaking at 655 nm) fades in the presence of glutathione (GSH). A sensitive and selective colorimetric GSH assay was worked out that has a linear response in the 10 to 0.1 µM GSH concentration range, with a 0.095 μM detection limit. The method was applied to the determination of GSH in spiked fetal calf serum where it gave excellent recoveries.

Schematic of the preparation of a nanohybrid composed of manganese dioxide and carbon based dots (MnO2/CDs). They can be used for the colorimetric detection of glutathione (GSH) based on the color change of 3,3′5,5′-tetramethylbenzidine (TMB).

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Acknowledgements

This study was financially supported by National Key Research and Development Program of China (2017YFC1600500), National Natural Science Foundation of China (21675026, 21675027, 21506029), Natural Science Foundation of Fujian Province (2017Y01010023).

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Authors and Affiliations

  1. MOE Key Laboratory of Analysis and Detection Technology for Food Safety, Fujian Provincial Key Laboratory of Analysis and Detection Technology for Food Safety, and Department of Chemistry, Fuzhou University, Fujian, China

    Qian Wang, Yongqiang Dong, Yuwu Chi & Fengfu Fu

  2. State Key Laboratory of Fine Chemicals, School of Chemical Engineering, Dalian University of Technology, Dalian, 116012, China

    Hongchang Pang

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  1. Qian Wang
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  2. Hongchang Pang
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Correspondence to Yongqiang Dong or Fengfu Fu.

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Wang, Q., Pang, H., Dong, Y. et al. Colorimetric determination of glutathione by using a nanohybrid composed of manganese dioxide and carbon dots. Microchim Acta 185, 291 (2018). https://doi.org/10.1007/s00604-018-2830-6

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