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A nanocomposite prepared from copper(II) and nitrogen-doped graphene quantum dots with peroxidase mimicking properties for chemiluminescent determination of uric acid

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Abstract

Nitrogen-doped graphene quantum dots (N-GQD) were employed along with Cu(II) ions under alkaline conditions and room temperature to synthesize nanocomposites of type Cu(II)/Cu2O/N-GQDs. These nanocomposites exhibit excellent stability and dispersity, and also display a peroxidase-like activity that is superior to pure Cu2O nanoparticles and natural peroxidase (POx). A chemiluminescence (CL) method was designed that is based on the use of uricase which oxidizes uric acid under formation of H2O2. The nanocomposites were used as a POx mimic in the luminol-H2O2 CL system. Under optimized conditions, a linear relationship between CL intensity and the uric acid (UA) concentration in the range of 0.16—4.0 μM, and a detection limit of 0.041 μM (at S/N = 3) were obtained. The CL method was applied to the determination of UA in spiked serum and urine, and recoveries ranged from 85.0 to 121.3%.

Schematic presentation of synthesis strategy of Cu(II)/Cu2O/N-GQDs and the CL method based Cu(II)/Cu2O/N-GQDs for H2O2-meidated uric acid detection. The method can be used for the determination of uric acid (UA) with the detection limit of 0.041 μM.

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Acknowledgements

This work was financially supported by the Natural Science Foundation of China (No. 21665001).

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Authors and Affiliations

  1. Key Laboratory of Regional Ecological Environment Analysis and Pollution Control of West Guangxi, College of Chemistry and Environmental Engineering, Baise University, Baise, 533000, China

    Bingfang Shi, Yan Duan, Shengyu Chen & Weiyuan Zuo

  2. State Key Laboratory for the Chemistry and Molecular Engineering of Medicinal Resources, School of Chemistry and Pharmaceutical Sciences, Guangxi Normal University, Guilin, 541004, China

    Yubin Su

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  1. Bingfang Shi
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  2. Yubin Su
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  3. Yan Duan
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  4. Shengyu Chen
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Correspondence to Bingfang Shi.

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Shi, B., Su, Y., Duan, Y. et al. A nanocomposite prepared from copper(II) and nitrogen-doped graphene quantum dots with peroxidase mimicking properties for chemiluminescent determination of uric acid. Microchim Acta 186, 397 (2019). https://doi.org/10.1007/s00604-019-3491-9

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