Abstracts
Guava (Psidium guajava L.), an important fruit crop of several tropical and sub-tropical countries, is facing several agronomic and horticultural problems such as susceptibility to many pathogens, particularly guava wilting caused by Fusarium oxysporium psidii, low fruit growth, short shelf life of fruits, high seed content, and stress sensitivity. Conventional breeding techniques have limited scope in improvement of guava owing to long juvenile period, self incompatibility, and heterozygous nature. Conventional propagation methods, i.e., cutting, grafting or stool layering, for improvement of guava already exist, but the long juvenile period has made them time consuming and cumbersome. Several biotechnological approaches such as genetic transformation may be effective practical solutions for such problems and improvement of guava. The improvement of fruit trees through genetic transformation requires an efficient regeneration system. During the past 2–3 decades, different approaches have been made for in vitro propagation of guava. An overview on the in vitro regeneration of guava via organogenesis, somatic embryogenesis, and synthetic seeds is presented. Organogenesis in several different genotypes through various explant selection from mature tree and seedling plants has been achieved. Factors affecting somatic embryogenesis in guava have been reviewed. Production of synthetic seeds using embryogenic propagules, i.e., somatic embryos and non-embryogenic vegetative propagules, i.e., shoot tips and nodal segments have also been achieved. Development of synthetic seed in guava may be applicable for propagation, short-term storage, and germplasm exchange, and distribution. An initial attempt for genetic transformation has also been reported. The purpose of this review is to focus upon the current information on in vitro propagation and biotechnological advances made in guava.
Similar content being viewed by others
References
Acosta M, Caballero I, Alvarado Y, Leiva M (2002) Epiphytic microbiota and fungal contaminants from in vitro establishment of guava (Psidium guajava L). Biotecnologia Vegetal 2:67–71
Akhtar N (1997) Studies on induction of somatic embryogenesis and production of artificial seeds for micropropagation of a tropical fruit tree guava (Psidium guajava L.). Ph.D. thesis, Banaras Hindu University, Varanasi, India
Akhtar N, Kumari N, Pandey S, Ara H, Singh M, Jaiswal U, Jaiswal VS, Jain SM (2000) Somatic embryogenesis in tropical fruit trees. In: Jain SM, Gupta PK, Newton RJ (eds) Somatic embryogenesis in woody plants, vol 6. Kluwer, Dordrecht, pp 93–140
Ali N, Mulwa RMS, Norton MA, Skirvin RM (2003) Micropropagation of guava (Psidium guajava L.). J Hort Sci Biotechnol 78:739–741
Ali N, Mulwa RMS, Norton MA, Skirvin RM (2007) Radical disinfestation protocol eliminates in vitro contamination in Guava (Psidium guajava L.) seeds. Plant Cell Tissue Organ Cult 91:295–298
Amin MN, Jaiswal VS (1987) Rapid clonal propagation of guava through in vitro shoot proliferation on nodal explants of mature trees. Plant Cell Tissue Organ Cult 9:235–243
Amin MN, Jaiswal VS (1988) Micropropagation as an aid to rapid cloning of a guava cultivar. Sci Hort 36:89–95
Amin MN, Jaiswal VS (1989a) In vitro propagation of guava (Psidium guajava L.): effects of sucrose, agar and pH on growth and proliferation of shoots. Bangladesh J Bot 18:1–8
Amin MN, Jaiswal VS (1989b) Effects of phloroglucinol, sucrose pH and temperature in vitro rooting of guava (Psidium guajava L.) microcutting. Bangladesh J Bot 18:129–139
Ara H, Jaiswal U, Jaiswal VS (2000) Plant regeneration from protoplasts of mango (Mangifera indica L.) through somatic embryogenesis. Plant Cell Rep 19:622–627
Ara H, Jaiswal U, Jaiswal VS (2004) An improved method of proliferation of proembryogenic calli of Mangifera indica L. var. Amrapali for scale-up of somatic embryo production. Indian J Biotechnol 3:229–234
Arnold SV, Sabala I, Bozhkov P, Dyachok J, Filonova L (2002) Developmental pathways of somatic embryogenesis. Plant Cell Tissue Organ Cult 69:233–249
Benson EE (2000) In vitro recalcitrance: an introduction. In Vitro Cell Dev Biol Plant 36:141–148
Bhojwani SS, Razdan MK (1996) Plant tissue culture: theory and practice. Elsevier, Amsterdam
Biswas BK, Yadav A, Joshee N, Yadav AK (2005) In vitro plant regeneration and genetic transformation to enhance cold hardiness in guava: a nutraceutical fruit. In: Proceeding of 1st international guava symposium. CISH, Lucknow, India, (abst.) pp 31–32
Biswas BK, Joshee N, Yadav A, Yadav AK (2007) Development and application of biotechnology in guava: a nutraceutical fruit. In: Desjardins Y (ed) ISHS Acta Horticulturae 744: Ist international symposium on human health effects of fruits and vegetables, pp 267–276
Chandra R, Bajpai A, Gupta S, Tiwari RK (2004) Embryogenesis and plant regeneration from mesocarp of Psidium guajava L. (guava). Indian J Biotech 3:246–248
Chandra R, Mishra M, Abida M, Singh DB (2005a) Paclobutrazol mediated somatic embryogenesis in guava. In: Abstract of 1st international guava symposium. CISH, Lucknow, India, p 34
Chandra R, Mishra M, Bajpai A (2005b) Biotechnological interventions for improvement of guava (Psidium guajava L.). In: Kishun R, Mishra AK, Singh G, Chandra R (eds) Proceeding of 1st international guava symposium. CISH, Lucknow, India, pp 19–25
Chen TW, Chang-Chai NG, CY Wang, Shyu YT (2007) Molecular identification and analysis of Psidium guajava L. from indigenous tribes of Taiwan. J Food Drug Anal 15:82–88
Christianson ML (1987) Causal events in morphogenesis. In: Green CE, Somers DA, Hackett WP, Biesboer DD (eds) Plant tissue and cell culture. A.R. Liss., New York, pp 44–55
Croft KPC, Jüttner F, Slusarenko AJ (1993) Volatile products of the lipoxygenase pathway evolved from Phaseolus vulgaris (L.) leaves inoculated with Pseudomonas syringae pv. phaseolicola. Plant Physiol 101:13–24
Dahiya KK, Archak S, Karihaloo JL (2002) DNA fingerprinting of guava (Psidium guajava L.) cultivars using RAPD markers. Indian J Plant Genet Resour 15:112–115
Dinesh MR, Iyer CPA (2005) Significant research achievement in guava—improvement and future needs. In: Kishun R, Mishra AK, Singh G, Chandra R (eds) Proceeding of 1st international guava symposium. CISH, Lucknow, India, pp 7–16
Doijode SD (2001) Guava: Psidium guajava L. In: Doijode SD (ed) Seed storage of horticultural crops. Haworth Press, New York, pp 65–67
Dudits D, Gyorgyey J, Bogre L, Bako L (1995) Molecular biology of somatic embryogenesis. In: Thorpe TA (ed) In vitro embryogenesis in plants. Kluwer Academic Publisher, Dordrecht, pp 267–308
Feria-Romero IA, Astudillo-Dela HV, Chavez-soto MA, Rivera-arce E, Lopez M, Serrano H, Lozoya X (2009) RAPD markers associated with quercetin accumulation in Psidium guajava. Bio Plant 53:125–128
Fuenmayor MED, Montero NJM (1997) In vitro clonal propagation of guava (Psidium guajava L.) from stem shoot of cv. Mara-7. Acta Hort 452:47–51
George EF (1993) Plant propagation by tissue culture: part 1. The technology, Exegetics, Edington, England
Giri C, Shyamkumar B, Anjaneyulu C (2004) Progress in tissue culture, genetic transformation and applications of biotechnology to trees: an overview. Trees Struct Funct 18:115–135
Gomez-Lim MA, Litz RE (2004) Genetic transformation of perennial tropical fruits. In Vitro Cell Dev Biol Plant 40:442–449
Gupta PK, Durzan DJ (1987) Biotechnology of somatic polyembryogenesis and plantlet regeneration in loblolly pine. Biotechnol 5:147–151
Hernandez-Delgado S, Padilla-Ramirez JS, Nava-Cedillo A, Mayek-Perez N (2007) Morphological and genetic diversity of Mexican guava germplasm. Plant Genet Resour 5:131–141
Jaiswal VS, Amin MN (1987) In vitro propagation of guava from shoot cultures of mature trees. J Plant Physiol 130:7–12
Jaiswal VS, Amin MN (1992) Guava and jackfruit. In: Hammerschlag FA, Litz RE (eds) Biotechnology of perennial fruit crops, Biotechnology in agriculture, vol 8. CAB International, Wallingford, pp 421–431
Jaiswal U, Jaiswal VS (2005) Psidium guajava. In: Litz RE (ed) Biotechnology of fruit and nut crops. CAB International, Wallingford, pp 394–401
Jaiswal VS, Akhtar N, Rai MK (2005) Role of different factors on somatic embryogenesis and synthetic seed production in guava. In: Abstract of 1st international guava symposium. CISH, Lucknow, India, p 36
Jimenez VM (2005) Involvement of plant hormones and plant growth regulators on in vitro somatic embryogenesis. Plant Growth Regul 47:91–110
Kim IS, Grosch W (1981) Partial-purification and properties of a hydroperoxide lyase from fruits of Pear. J Agric Food Chem 29:1220–1225
Komamine A, Murata N, Nomura K (2005) Mechanisms of somatic embryogenesis in carrot suspension cultures—morphology, physiology, biochemistry, and molecular biology. In vitro Cell Dev Biol Plant 41:6–10
Kosky RG, Perozo JV, Valero NA, Penalver DA (2005) Somatic embryo germination of Psidium guajava L. in the Rita® temporary immersion system, on semisolid medium. In: Hvoslef-Eide KA, Preil W (eds) Liquid culture systems for in vitro plant propagation. Springer-Verlag, New York, pp 225–229
Krishna H, Singh SK (2007) Biotechnological advances in mango (Mangifera indica L.) and their future implication in crop improvement—a review. Biotech Adv 25:223–243
Litz RE, Gray DJ (1992) Organogenesis and somatic embryogenesis. In: Hammerschlag FA, Litz RE (eds) Biotechnology of perennial fruit crops. CAB International, Wallingford, pp 3–34
Litz RE, Jaiswal VS (1991) Micropropagation of tropical and subtropical fruits. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Kluwer Academic Publishers, Dordrecht, pp 247–263
Loh CS, Rao AN (1989) Clonal propagation of guava (Psidium guajava L.) from seedlings and grafted plants and adventitious shoot formation in vitro. Sci Hort 39:31–39
Malik SK, Chaudhury R, Kalia RK (2005) Rapid in vitro multiplication and conservation of Garcinia indica: a tropical medicinal tree species. Sci Hort 106:539–553
Meghwal PR, Singh SK, Sharma HC (2003) Micropropagation of Aneuploid guava. Indian J Hort 60:29–33
Mishra AK (2005) Important diseases of guava in India with special reference to wilt. In: Kishun R, Mishra AK, Singh G, Chandra R (eds) Proceeding of 1st international guava symposium. CISH, Lucknow, India, pp 75–90
Mishra DS, Tiwari JP, Lal S (2005) In vitro cloning of guava (Psidium guajava L.) cv. Pant Prabahat. In: Abstract of 1st international guava symposium. CISH, Lucknow, India, pp 32–33
Murashige T, Skoog FA (1962) Revised medium for rapid growth and bioassays with tobacco tissue cultures. Physiol Plant 15:473–497
Papadatau P, Pontikis CA, Ephtimiadou E, Lydaki M (1990) Rapid multiplication of guava seedlings by in vitro shoot tip culture. Sci Hort 45:99–108
Pati PK, Rath SP, Sharma M, Sood A, Ahuja PS (2006) In vitro propagation of rose—a review. Biotechnol Adv 24:94–114
Pommer CV, Murakami KRN (2008) Breeding guava (Psidium guajava L.). In: Jain SM, Priyadarshan PM (eds) Breeding plantation tree crops: tropical species. Springer, New York, pp 83–119
Porebski S, Bailey LG, Baurn BR (1997) Modification of a CTAB DNA extraction protocol for plants containing high polysaccharide and polyphenol components. Plant Mol Biol Reprod 15:8–15
Prakash DP, Narayanaswamy P, Sondur SN (2002) Analysis of molecular diversity in Guava using RAPD markers. J Hort Sci Biotechnol 77:287–293
Purohit M, Srivastava S, Srivastava PS (1998) Stress tolerant plants through tissue culture. In: Srivastava PS (ed) Plant tissue culture and molecular biology: application and prospects. Narosa Publishing House, New Delhi, pp 554–578
Rai MK, Jaiswal VS (2008) Synthetic seeds of guava (Psidium guajava L) from somatic embryos and plant regeneration. In: Arya ID, Arya S (eds) Utilization of biotechnology in plant sciences. Microsoft Printech (I) Pvt Ltd, Dehradun, pp 217–222
Rai MK, Akhtar N, Jaiswal VS (2007) Somatic embryogenesis and plant regeneration in Psidium guajava L. cv. Banarasi local. Sci Hort 113:129–133
Rai MK, Jaiswal VS, Jaiswal U (2008a) Effect of ABA and sucrose on germination of encapsulated somatic embryos of guava (Psidium guajava L.). Sci Hort 117:302–305
Rai MK, Jaiswal VS, Jaiswal U (2008b) Encapsulation of shoot tips of guava (Psidium guajava L.) for short-term storage and germplasm exchange. Sci Hort 118:33–38
Rai MK, Jaiswal VS, Jaiswal U (2008c) Alginate-encapsulation of nodal segments of guava (Psidium guajava L.) for germplasm exchange and distribution. J Hort Sci Biotechnol 83:569–573
Rai MK, Jaiswal VS, Jaiswal U (2009a) Effect of selected amino acids and polyethylene glycol on maturation and germination of somatic embryos of guava (Psidium guajava L.). Sci Hort 121:233–236
Rai MK, Jaiswal VS, Jaiswal U (2009b) Shoot multiplication and plant regeneration of guava (Psidium guajava L.) from nodal explants of in vitro raised plantlets. J Fruit Ornam Plant Res 17:29–38
Raj Bhansali R (1990) Somatic embryogenesis and regeneration of plantlets in pomegranate. Ann Bot 66:249–253
Ribeiro IJA, Pommer CV (2004) Breeding guava (Psidium guajava) for resistance to rust caused by Puccinia psidii. Acta Hort 632:75–78
Risterucci AM, Duval MF, Rohde W, Billotte N (2005) Isolation and characterization of microsatellite loci from Psidium guajava L. Mol Ecol Notes 5:745–748
Rout GR, Samantaray S, Das P (2000) In vitro manipulation and propagation of medicinal plants. Biotechnol Adv 18:91–120
Schroeder CA (1961) Some morphological aspects of fruit tissues grown in vitro. Bot Gaz 122:198–204
Shah ST, Zamir R, Ahmad J, Ali H, Lutfullah G (2008) In vitro regeneration of plantlets from seedling explants of guava (Psidium guajava L.) cv. Safeda. Pak J Bot 40:1195–1200
Sharp WR, Sondahl MR, Caldas LS, Maraffa SB (1980) The physiology of in vitro asexual embryogenesis. Hortic Rev 268–310
Singh G (2005) Strategies for improved production in guava. In: Kishun R, Mishra AK, Singh G, Chandra R (eds) Proceeding of 1st international guava symposium. CISH, Lucknow, India, pp 26–39
Singh SK, Meghwal PR, Sharma HC, Singh SP (2002) Direct shoot organogenesis on explants from germinated seedlings of Psidium guajava L. cv. Allahabad Safeda. Sci Hort 95:213–221
Singh M, Jaiswal U, Jaiswal VS (2004) In vitro regeneration and improvement in tropical fruit trees: an assessment. In: Srivastava PS, Narula A, Srivastava S (eds) Plant biotechnology and molecular markers. Anamanya Publishers, New Delhi, pp 228–243
Singh D, Mohammed S, Shukla AK (2005) Micropropagation of guava as influenced by season, source and pretreatment to explant. In: Abstract of 1st international guava symposium. CISH, Lucknow, India, p 33
Srivastava U (2005) Genetic resources management in guava. In: Kishun R, Mishra AK, Singh G, Chandra R (eds) Proceeding of 1st international guava symposium. CISH, Lucknow, India, pp 17–18
Stasolla C, Yeung EC (2003) Recent advances in conifer somatic embryogenesis: improving somatic embryo quality. Plant Cell Tissue Organ Cult 74:15–35
Thomas TD (2008) The role of activated charcoal in plant tissue culture. Biotechnol Adv 26:618–631
Thorpe TA, Harpy IS, Kumar PP (1991) Application of micropropagation to forestry. In: Debergh PC, Zimmerman RH (eds) Micropropagation. Kluwer Academic Publishers, Dordrecht, pp 311–336
Tijet N, Waspi U, Gaskin DJH, Hunziker P, Muller BL, Vulfson EN, Slusarenko A, Brash AR, Whitehead IM (2000) Purification, molecular cloning, and expression of the gene encoding fatty acid 13-hydroperoxide lyase from guava fruit (Psidium guajava). Lipids 35:709–720
Yasseen M, Barringer SA, Schnell RJ, Splittoesser WE (1995) In vitro shoot proliferation of guava (Psidium guajava L.) from germinated seedlings. Plant Cell Rep 14:525–528
Zamir R, Khattak GSS, Mohammad T, Ali N (2003) In vitro mutagenesis in guava (Psidium guajava L.). Pak J Bot 35:825–828
Zamir R, Ali N, Shah ST, Muhammad T, Shah SA (2007) In vitro regeneration of guava (Psidium guajava L.) from shoot tips of mature trees. Pak J Bot 39:2395–2398
Zimmerman JL (1993) Somatic embryogenesis: a model of early development in higher plants. Plant Cell 5:1411–1423
Acknowledgments
Financial assistance provided by Council of Scientific & Industrial Research (CSIR), New Delhi, to the authors (MKR and PA) is gratefully acknowledged. Suggestions by the anonymous reviewers for improving the manuscript are also very much appreciated.
Author information
Authors and Affiliations
Corresponding author
Additional information
Communicated by J. Carlson.
Rights and permissions
About this article
Cite this article
Rai, M.K., Asthana, P., Jaiswal, V.S. et al. Biotechnological advances in guava (Psidium guajava L.): recent developments and prospects for further research. Trees 24, 1–12 (2010). https://doi.org/10.1007/s00468-009-0384-2
Received:
Revised:
Accepted:
Published:
Issue Date:
DOI: https://doi.org/10.1007/s00468-009-0384-2