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Cross-linked enzyme aggregates (CLEAs) of cellulase with improved catalytic activity, adaptability and reusability

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Abstract

In this study, cross-linked cellulase aggregates (C-CLEAs) were synthesized by precipitation of cellulase with ammonium sulfate and then cross-linking with glutaraldehyde. The results revealed that the optimal pH of C-CLEAs shifted toward a more acidic environment by 2.0 pH units, and the optimal temperature shifted toward higher temperature by 20 °C after immobilization. The half-life (t1/2) and inactivation energy (Ed) values of the C-CLEAs were 5.98 times and 1.93 times than that of free cellulase, respectively. Moreover, the C-CLEAs can also maintain about 65.22% of activity after 10 cycles and 63.03% of activity after storage for 56 days at 4 °C. Enzymatic hydrolysis of carboxymethylcellulose sodium and corncob in C-CLEAs system verified that the C-CLEAs performed better than free cellulase (P < 0.01).

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Acknowledgements

We gratefully acknowledge financial support provided by National Natural Science Foundation of China (Grant no. 21464011) and General Science and Technology Project of “Double First Class University Plan” Construction of Shihezi University (SHYL-DK201803).

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Authors and Affiliations

  1. Key Laboratory for Green Processing of Chemical Engineering of Xinjiang Bingtuan, School of Chemistry and Chemical Engineering, Shihezi University, Beisi Road, Shihezi, 800032, Xinjiang, China

    Tiantian Li, Xiaowu Gong, Gaoshan Yang, Qin Li, Jin Huang, Na Zhou & Xin Jia

  2. School of Chemistry and Chemical Engineering, and Chongqing Key Laboratory of Soft-Matter Material Chemistry and Functional Manufacturing, and “the Belt and Road (B&R)” International Joint Research Laboratory of Sustainable Materials, Southwest University, Chongqing, China

    Jin Huang

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  1. Tiantian Li
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  2. Xiaowu Gong
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  4. Qin Li
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  5. Jin Huang
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Li, T., Gong, X., Yang, G. et al. Cross-linked enzyme aggregates (CLEAs) of cellulase with improved catalytic activity, adaptability and reusability. Bioprocess Biosyst Eng 45, 865–875 (2022). https://doi.org/10.1007/s00449-022-02704-0

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