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Strategies to Reuse Cellulase: Immobilization of Enzymes (Part II)

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Approaches to Enhance Industrial Production of Fungal Cellulases

Part of the book series: Fungal Biology ((FUNGBIO))

Abstract

Industrial applications need enzymes that are economically viable and highly stable in terms of reusability, thus increasing practicability. The immobilization of cellulases is reported here using different chemical methods and polymeric supports. High costs of cellulases are one of the many obstacles for commercialization of biomass biorefineries. Cellulase immobilization allows the conditions of use of enzyme again and again retaining its activity and reducing production costs to use it for industrial application. Enzyme immobilization is accomplished by adsorption, entrapment, covalent binding, cross-linking, and encapsulation. Support material acts as a carrier for immobilized enzyme, having mechanical strength, large surface area, resistance to microbial attack, and many surface groups promoting interaction with enzyme. One procedure where no support is used is the formation of cross-linked enzyme aggregates (CLEA) in which enzyme cross-links with other enzyme-forming insoluble aggregate.

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Author information

Authors and Affiliations

  1. Department of Biotechnology, University of Sargodha, Sargodha, Pakistan

    Muhammad Irfan & Misbah Ghazanfar

  2. Institute of Bioprocess Engineering and Pharmaceutical Technology, Mittelhessen University of Applied Sciences, Giessen, Germany

    Amad Ur Rehman

  3. Physics of Surfaces, Center of Smart Interfaces, Technical University Darmstadt, Alarich-Weiss-Str., 1664287, Darmstadt, Germany

    Asma Siddique

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  1. Muhammad Irfan
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  2. Misbah Ghazanfar
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  3. Amad Ur Rehman
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  4. Asma Siddique
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Editor information

Editors and Affiliations

  1. Department of Physics and Astrophysics, University of Delhi, Delhi, Delhi, India

    Manish Srivastava

  2. Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University) Varanasi, Varanasi, Uttar Pradesh, India

    Neha Srivastava

  3. Department of Biological Sciences, Sam Higginbottom University of Agriculture, Technology and Sciences (SHUATS), Allahabad, Uttar Pradesh, India

    Pramod W. Ramteke

  4. Department of Chemical Engineering and Technology, Indian Institute of Technology (Banaras Hindu University) Varanasi, Varanasi, Uttar Pradesh, India

    Pradeep Kumar Mishra

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Irfan, M., Ghazanfar, M., Ur Rehman, A., Siddique, A. (2019). Strategies to Reuse Cellulase: Immobilization of Enzymes (Part II). In: Srivastava, M., Srivastava, N., Ramteke, P., Mishra, P. (eds) Approaches to Enhance Industrial Production of Fungal Cellulases . Fungal Biology. Springer, Cham. https://doi.org/10.1007/978-3-030-14726-6_9

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