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Improving the catalytic potential and substrate tolerance of Gibberella intermedia nitrilase by whole-cell immobilization

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Abstract

Comparative studies of immobilized and free cells of Gibberella intermedia CA3-1 in bioconversion of 3-cyanopyridine to nicotinic acid were performed. Entrapping method was chosen based on the advantages in enzymatic activity recovery, mechanical strength and preparation procedure. Four entrapment matrices were investigated and sodium alginate was screened to be the most suitable material. Maximal nitrilase activity of alginate immobilized cells was obtained under conditions of 2 % alginate, 0.6 % CaCl2, 0.4 g cell/g alginate, 1.8 mm bead size. The immobilized cells showed excellent substrate tolerance even when the 3-cyanopyridine concentration was 700 mM. The half-lives of immobilized cells at 30, 40 and 50 °C were 315, 117.5 and 10.9 h, respectively, correspondingly 1.4, 1.6 and 1.7-fold compared with that of the free cells. Efficient reusability of immobilized cells up to 28 batches was achieved and 205.7 g/(g dcw) nicotinic acid was obtained with 80.55 % enzyme activity preserved.

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Acknowledgments

This work was supported by the National Natural Science Foundation of China (21206055), Natural Science Foundation of Jiangsu Province (BK2012127, BK20140133), and National High Technology Research and Development Program of China (No. 2012AA022204C).

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Authors and Affiliations

  1. School of Pharmaceutical Science, Jiangnan University, 1800 Lihu Avenue, Wuxi, 214122, People’s Republic of China

    Heng Li, Tao Yang, Jin-Song Gong, Lei Xiong, Zhen-Ming Lu, Hui Li, Jin-Song Shi & Zheng-Hong Xu

  2. The Key Laboratory of Industrial Biotechnology, Ministry of Education, Jiangnan University, Wuxi, 214122, People’s Republic of China

    Jin-Song Gong & Zheng-Hong Xu

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  1. Heng Li
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  2. Tao Yang
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  3. Jin-Song Gong
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  6. Hui Li
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Correspondence to Zheng-Hong Xu.

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H. Li and T. Yang have contributed equally to this article.

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Li, H., Yang, T., Gong, JS. et al. Improving the catalytic potential and substrate tolerance of Gibberella intermedia nitrilase by whole-cell immobilization. Bioprocess Biosyst Eng 38, 189–197 (2015). https://doi.org/10.1007/s00449-014-1258-6

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  • DOI: https://doi.org/10.1007/s00449-014-1258-6

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