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An improved nitrilase-mediated bioprocess for synthesis of nicotinic acid from 3-cyanopyridine with hyperinduced Nocardia globerula NHB-2

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Journal of Industrial Microbiology & Biotechnology

Abstract

Nitrilase of Nocardia globerula NHB-2 was induced by short-chain aliphatic nitriles (valeronitrile > isobutyronitrile > butyronitrile > propionitrile) and exhibited activity towards aromatic nitriles (benzonitrile > 3-cyanopyridine > 4-cyanopyridine > m-tolunitrile > p-tolunitrile). Hyperinduction of nitrilase (6.67 U mg −1DCW , 18.7 U mL−1) was achieved in short incubation time (30 h, 30°C) through multiple feeding of isobutyronitrile in the growth medium. The nitrilase of this organism exhibits both substrate and product inhibition effects. In a fed batch reaction at 1 L scale using hyperinduced resting cells corresponding to 10 U mL−1 nitrilase activity (1.5 mgDCW mL−1), a total of 123.11 g nicotinic acid was produced at a rate of 24 g h−1 g −1DCW .

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Acknowledgments

The authors acknowledge the Council of Scientific and Industrial Research, New Delhi for financial support in the form of Senior Research Fellowship to Mr Nitya Nand Sharma and Ms Monica Sharma. The computational facility availed at Bioinformatics Centre, H P University is also duly acknowledged. The authors also thank Jubilant Organosis (India) for the gift of 3-CP.

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Authors and Affiliations

  1. Department of Biotechnology, Himachal Pradesh University, Summer Hill, Shimla, 171005, India

    Nitya Nand Sharma, Monica Sharma & Tek Chand Bhalla

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  1. Nitya Nand Sharma
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  2. Monica Sharma
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  3. Tek Chand Bhalla
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Correspondence to Tek Chand Bhalla.

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Sharma, N.N., Sharma, M. & Bhalla, T.C. An improved nitrilase-mediated bioprocess for synthesis of nicotinic acid from 3-cyanopyridine with hyperinduced Nocardia globerula NHB-2. J Ind Microbiol Biotechnol 38, 1235–1243 (2011). https://doi.org/10.1007/s10295-010-0902-7

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  • DOI: https://doi.org/10.1007/s10295-010-0902-7

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