Abstract
Aspergillus terricola and Aspergillus ochraceus, isolated from Brazilian soil, were cultivated in Vogel and Adams media supplemented with 20 different carbon sources, at 30 °C, under static conditions, for 120 and 144 h, respectively. High levels of cellulase-free xylanase were produced in birchwood or oat spelt xylan-media. Wheat bran was the most favorable agricultural residue for xylanase production. Maximum activity was obtained at 60 °C and pH 6.5 for A. terricola, and 65 °C and pH 5.0 for A. ochraceus. A. terricola xylanase was stable for 1 h at 60 °C and retained 50% activity after 80 min, while A. ochraceus xylanase presented a t50 of 10 min. The xylanases were stable in an alkali pH range. Biobleaching of 10 U/g dry cellulose pulp resulted in 14.3% delignification (A. terricola) and 36.4% (A. ochraceus). The brightness was 2.4–3.4% ISO higher than the control. Analysis in SEM showed defibrillation of the microfibrils. Arabinase traces and β-xylosidase were detected which might act synergistically with xylanase.
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Acknowledgments
This work was supported by the Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and the Conselho de Desenvolvimento Científico e Tecnológico (CNPq). J.A.J., H.F.T., and M.L.T.M.P. are research fellows of CNPq. M.M. is a recipient of a FAPESP fellowship, and this work is part of her doctoral thesis. We thank Ricardo Alarcon and Maurício de Oliveira for technical assistance.
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Michelin, M., Peixoto-Nogueira, S.C., Betini, J.H.A. et al. Production and properties of xylanases from Aspergillus terricola Marchal and Aspergillus ochraceus and their use in cellulose pulp bleaching. Bioprocess Biosyst Eng 33, 813–821 (2010). https://doi.org/10.1007/s00449-009-0403-0
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DOI: https://doi.org/10.1007/s00449-009-0403-0