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Production of xylanase by Aspergilli using alternative carbon sources: application of the crude extract on cellulose pulp biobleaching

  • Original Paper
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Journal of Industrial Microbiology & Biotechnology

Abstract

The ability of xylanolytic enzymes produced by Aspergillus fumigatus RP04 and Aspergillus niveus RP05 to promote the biobleaching of cellulose pulp was investigated. Both fungi grew for 4–5 days in liquid medium at 40°C, under static conditions. Xylanase production was tested using different carbon sources, including some types of xylans. A. fumigatus produced high levels of xylanase on agricultural residues (corncob or wheat bran), whereas A. niveus produced more xylanase on birchwood xylan. The optimum temperature of the xylanases from A. fumigatus and A. niveus was around 60–70°C. The enzymes were stable for 30 min at 60°C, maintaining 95–98% of the initial activity. After 1 h at this temperature, the xylanase from A. niveus still retained 85% of initial activity, while the xylanase from A. fumigatus was only 40% active. The pH optimum of the xylanases was acidic (4.5–5.5). The pH stability for the xylanase from A. fumigatus was higher at pH 6.0–8.0, while the enzyme from A. niveus was more stable at pH 4.5–6.5. Crude enzymatic extracts were used to clarify cellulose pulp and the best result was obtained with the A. niveus preparation, showing kappa efficiency around 39.6% as compared to only 11.7% for that of A. fumigatus.

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Acknowledgments

This work was supported by Fundação de Amparo à Pesquisa do Estado de São Paulo (FAPESP) and Conselho Nacional de Desenvolvimento Científico e Tecnológico (CNPq). J. A. J., H.·F. T. and M. L. T. M. P. are Research Fellows of CNPq. S.·C. P.·N. was a recipient of a CNPq fellowship and this study is part of her Doctoral Thesis. M. M. was a recipient of a FAPESP fellowship. The authors thank Ricardo F. Alarcon and Mauricio de Oliveira for technical assistance.

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Authors and Affiliations

  1. Departamento de Bioquímica e Imunologia, Faculdade de Medicina de Ribeirão Preto, Universidade de São Paulo, Ribeirão Preto, Brazil

    Simone de Carvalho Peixoto-Nogueira

  2. Departamento de Biologia, Faculdade de Filosofia, Ciências e Letras de Ribeirão Preto, Universidade de São Paulo, Avenida Bandeirantes 3900, Monte Alegre, Ribeirão Preto, SP, 14.040-901, Brazil

    Michele Michelin, Jorge Henrique Almeida Betini, João Atílio Jorge, Héctor Francisco Terenzi & Maria de Lourdes Teixeira de Moraes Polizeli

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  1. Simone de Carvalho Peixoto-Nogueira
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  2. Michele Michelin
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  3. Jorge Henrique Almeida Betini
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Correspondence to Maria de Lourdes Teixeira de Moraes Polizeli.

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de Carvalho Peixoto-Nogueira, S., Michelin, M., Betini, J.H.A. et al. Production of xylanase by Aspergilli using alternative carbon sources: application of the crude extract on cellulose pulp biobleaching. J Ind Microbiol Biotechnol 36, 149–155 (2009). https://doi.org/10.1007/s10295-008-0482-y

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