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Genetic analysis of durable resistance to Magnaporthe oryzae in the rice accession Gigante Vercelli identified two blast resistance loci

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Abstract

Rice cultivars exhibiting durable resistance to blast, the most important rice fungal disease provoking up to 30 % of rice losses, are very rare and searching for sources of such a resistance represents a priority for rice-breeding programs. To this aim we analyzed Gigante Vercelli (GV) and Vialone Nano (VN), two temperate japonica rice cultivars in Italy displaying contrasting response to blast, with GV showing a durable and broad-spectrum resistance, whereas VN being highly susceptible. An SSR-based genetic map developed using a GV × VN population segregating for blast resistance identified two blast resistance loci, localized to the long arm of chromosomes 1 and 4 explaining more than 78 % of the observed phenotypic variation for blast resistance. The pyramiding of two blast resistance QTLs was therefore involved in the observed durable resistance in GV. Mapping data were integrated with information obtained from RNA-seq expression profiling of all classes of resistance protein genes (resistance gene analogs, RGAs) and with the map position of known cloned or mapped blast resistance genes to search candidates for the GV resistant response. A co-localization of RGAs with the LOD peak or the marker interval of the chromosome 1 QTL was highlighted and a valuable tool for selecting the resistance gene during breeding programs was developed. Comparative analysis with known blast resistance genes revealed co-positional relationships between the chromosome 1 QTL with the Pi35/Pish blast resistance alleles and showed that the chromosome 4 QTL represents a newly identified blast resistance gene. The present genetic analysis has therefore allowed the identification of two blast resistance loci in the durable blast-resistant rice cultivar GV and tools for molecular selection of these resistance genes.

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Acknowledgments

This research was supported by the Project “AGER RISINNOVA” (AGER grant no 2010-2369) and by the Project “ESPLORA” (MiPAAF DM 14658-7303). The authors wish to thank Gabriele Orasen and Ivana Tagliaferri for plants management.

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  1. Federica Assenza

    Present address: Plant Biochemistry, Institute of Agricultural Sciences, ETH Zürich, LFW D 38 Universitätstrasse 2, 8092, Zurich, Switzerland

  2. Giada Guarnieri

    Present address: Continental Semences S.p.A., via Monzato 9, 43029, Traversetolo, PR, Italy

Authors and Affiliations

  1. Council for Agricultural Research and Economics (CRA), Genomics Research Centre, Via S. Protaso, 302, 29017, Fiorenzuola d’Arda, Piacenza, Italy

    Simona Urso, Francesca Desiderio, Chiara Biselli, Paolo Bagnaresi, Federica Assenza, Giada Guarnieri, Luigi Cattivelli & Giampiero Valè

  2. Council for Agricultural Research and Economics (CRA), Rice Research Unit, S.S. 11 to Torino, Km 2,5, 13100, Vercelli, Italy

    Chiara Biselli & Giampiero Valè

  3. Rice Genomics Group, Parco Tecnologico Padano, Via Einstein, 26900, Lodi, Italy

    Laura Crispino, Pietro Piffanelli & Pamela Abbruscato

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  1. Simona Urso
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Correspondence to Giampiero Valè.

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Urso, S., Desiderio, F., Biselli, C. et al. Genetic analysis of durable resistance to Magnaporthe oryzae in the rice accession Gigante Vercelli identified two blast resistance loci. Mol Genet Genomics 291, 17–32 (2016). https://doi.org/10.1007/s00438-015-1085-8

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