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Pi35(t), a new gene conferring partial resistance to leaf blast in the rice cultivar Hokkai 188

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Abstract

The japonica rice cultivar Hokkai 188 shows a high level of partial resistance to leaf blast. For mapping genes conferring the resistance, a set of 190 F2 progeny/F3 families was developed from the cross between the indica rice cultivar Danghang-Shali, with a low level of partial resistance, and Hokkai 188. Partial resistance to leaf blast in the F3 families was assessed in upland nurseries. From a primary microsatellite (SSR) linkage map and QTL analysis using a subset of 126 F2 progeny/F3 families randomly selected from the above set, one major QTL located on chromosome 1 was detected in the vicinity of SSR marker RM1216. This QTL was responsible for 69.4% of the phenotypic variation, and Hokkai 188 contributed the resistance allele. Segregation analysis in the F3 families for partial resistance to leaf blast was in agreement with the existence of a major gene, and the gene was designated as Pi35(t). Another QTL detected on chromosome 8 was minor, explained 13.4% of the phenotypic variation, and an allele of Danghang-Shali increased the level of resistance in this QTL. Additional SSR markers of the targeted Pi35(t) region were further surveyed in the 190 F2 plants, and Pi35(t) was placed in a 3.5-cM interval flanked by markers RM1216 and RM1003.

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Acknowledgments

We would like to thank Dr. Yano, M. (National Institute for Agricultural Science—NIAS, Japan) for providing the SSR primers for parental survey. We gratefully acknowledge technical assistance of Dr. Kawasaki, S. (NIAS, Japan). We also thank Dr. Fukuoka, M. (NIAS, Japan), Dr. Nguyen, B. (Texas Tech University, USA) and Dr. Takeuchi, Y. (National Institute of Crop Science, Japan) for their helpful advices in the data analyses. This work was partially supported by the grant from Japan Society for Promotion of Science.

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Authors and Affiliations

  1. Fungal Disease Laboratory, Department of Plant Pathology, National Agricultural Research Organization, Kannondai 3-1-1, Tsukuba, Ibaraki, 305-8666, Japan

    T. T. T. Nguyen, S. Koizumi, T. N. La, N. Yasuda, I. Imazaki & A. Miyasaka

  2. Molecular Biology Department, Agricultural Genetics Institute, Hanoi, Vietnam

    T. T. T. Nguyen & T. N. La

  3. National Agricultural Research Center for Tohoku Region, Yotsuya, Omagari, Akita, 014-0102, Japan

    K. S. Zenbayashi & T. Ashizawa

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  1. T. T. T. Nguyen
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  2. S. Koizumi
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  3. T. N. La
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  4. K. S. Zenbayashi
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  5. T. Ashizawa
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  6. N. Yasuda
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  7. I. Imazaki
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  8. A. Miyasaka
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Correspondence to S. Koizumi.

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Communicated by Q. Zhang

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Nguyen, T.T.T., Koizumi, S., La, T.N. et al. Pi35(t), a new gene conferring partial resistance to leaf blast in the rice cultivar Hokkai 188. Theor Appl Genet 113, 697–704 (2006). https://doi.org/10.1007/s00122-006-0337-8

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  • DOI: https://doi.org/10.1007/s00122-006-0337-8

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