Abstract
In Sicily, small differences exist between wild and cultivated rosemary biotypes; VOCs and genetic profiles may be a useful tool to distinguish them. A germplasm collection of Rosmarinus officinalis L. was harvested from 15 locations in Sicily. Eleven wild and four cultivated populations were collected and, due to the surveyed area covered, they can be considered as a representative panel of Sicilian genetic background of the species. Ex situ plant collection was transferred to the field cultivation in homogeneous conditions for characterizing through a multidisciplinary approach. The study included morphological traits observations (growth habitus, flower color, number and size of leaves, length and number of internodes), VOC profiles using HS-SPME, genome size by flow cytometry analysis, and genetic characterization by means of DNA and nuclear microsatellite (nSSR) investigation. To detect any pattern within- and among-populations variability, all morphological and chemical data were submitted to ANOVA, while clustering and structure population analysis were carried out using genetic profiles. The present work allowed us to distinguish rather well between wild and cultivated genotypes and to underline the biodiversity richness among rosemary Sicilian germplasm, never highlighted, useful for future breeding programs addressed to exploit this important resource.
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References
Alipour M, Saharkhiz MJ (2016) Phytotoxic activity and variation in essential oil content and composition of rosemary (Rosmarinus officinalis L.) during different phenological growth stages. Biocatal Agric Biotechnol 7:271–278
Andrade JM, Faustino C, Garcia C, Ladeiras D, Reis CP, Rijo P (2018) Rosmarinus officinalis L: an update review of its phytochemistry and biological activity. Future Sci OA 4:FSO283
Angioni A, Barra A, Cereti E, Barile D, Coisson JD, Arlorio M, Dessi S, Coroneo V, Cabras P (2004) Chemical composition, plant genetic differences, antimicrobial and antifungal activity investigation of the essential oil of Rosmarinus officinalis L. J Agric Food Chem 52:3530–3535
Araniti F, Lupini A, Mercati F, Statti GA, Abenavoli MR (2013) Calamintha nepeta L. (Savi) as source of phytotoxic compounds: bio-guided fractionation in identifying biological active molecules. Acta Physiol Plant 35:1979–1988. https://doi.org/10.1007/s11738-013-1236-7
Araniti F, Marrelli M, Lupini A, Mercati F, Statti GA, Abenavoli MR (2014) Phytotoxic activity of Cachrys pungens Jan, a Mediterranean species: separation, identification and quantification of potential allelochemicals. Acta Physiol Plant 36:1071–1083. https://doi.org/10.1007/s11738-013-1482-8
Atak M, Mavi K, Uremis I (2016) Bio-herbicidal effects of oregano and rosemary essential oils on germination and seedling growth of bread wheat cultivars and weeds. Rom Biotechnol Lett 21:11149–11159
Bennetzen JL, Ma J, Devos KM (2005) Mechanisms of recent genome size variation in flowering plants. Ann Bot 95:127–132
Burkhart EP, Jacobson MG (2009) Transitioning from wild collection to forest cultivation of indigenous medicinal forest plants in eastern North America is constrained by lack of profitability. Agrofor Syst 76:437–453
Bruvo R, Michiels NK, D'Souza TG, Schulenburg H (2004) A simple method for the calculation of microsatellite genotype distances irrespective of ploidy level. Mol Ecol 13:2101–2106
Carimi F, Mercati F, De Michele R, Fiore MC, Riccardi P, Sunseri F (2011) Intra-varietal genetic diversity of the grapevine (Vitis vinifera L.) cultivar ‘Nero d'Avola’ as revealed by microsatellite markers. Genet Res Crop Evol 58(7):967–975. https://doi.org/10.1007/s10722-011-9731-4
Carrillo JD, Tena MT (2006) Determination of volatile compounds in antioxidant rosemary extracts by multiple headspace solid-phase microextraction and gas chromatography. Flavour Fragr J 21:626–633. https://doi.org/10.1002/ffj.1630
Carrubba A, Ascolillo V, Pagan Domenech AT, Saiano F, Aiello P (2009) Modifications over time of volatile compounds in Coriander (Coriandrum sativum L). Acta Hort 826:43–49
Carrubba A, Militello M, Saiano F, Pagan Domenech AT (2011) Comparison between different techniques for volatiles analyses in Coriander (Coriandrum sativum L.). Acta Hort 925:151–154
Chagné D (2015) Application of the high-resolution melting technique for gene mapping and SNP detection in plants. Methods Mol Biol 1245:151–159. https://doi.org/10.1007/978-1-4939-1966-6_11
CREA (2013) PlantA-Res. Rete Nazionale delle Risorse Genetiche Vegetali per l'Alimentazione e l'Agricoltura. Piante Aromatiche e Medicinali. Rosmarino. https://planta-res.politicheagricole.it/schede_descr/ROSMARINO.pdf. Accessed 10 Sep 2019
D’Auria M, Racioppi R (2015) The effect of drying on the composition of volatile organic compounds in Rosmarinus officinalis, Laurus nobilis, Salvia officinalis and Thymus serpyllum. A HS-SPME-GC-MS study. J Essent Oil Bear Pl 5:1209–1223. https://doi.org/10.1080/0972060X.2014.895213
De Mastro G, Ruta C, Mincione A, Poiana M (2004) Bio-morphological and chemical characterization of rosemary (Rosmarinus officinalis L.) biotypes. Acta Hort 629:471–482
Dolezel J, Bartos J (2005) Plant DNA flow cytometry and estimation of nuclear genome size. Ann Bot 95:99–110
Dolezel J, Greilhuber J, Suda J (2007) Flow cytometry with plant cells. Analysis of genes, chromosomes and genomes. Wiley, Weinheim
Drew BT, González‐Gallegos JG, Xiang C-L, Kriebel R, Drummond CP, Walker JB, Sytsma KJ (2017) Salvia united: the greatest good for the greatest number. Taxon 66(1):133–145. https://doi.org/10.12705/661.7
Euro+Med (2018) Rosmarinus. https://ww2.bgbm.org/EuroPlusMed/. Accessed 10 Sep 2019
Evanno G, Regnaut S, Goudet J (2005) Detecting the number of clusters of individuals using the software STRUCTURE: a simulation study. Mol Ecol 14:2611–2620
Ferrer-Gallego P, Ferrer-Gallego R, Roselló R, Peris JB, Guillén A, Gómez J, Laguna E (2014) A new subspecies of Rosmarinus officinalis (Lamiaceae) from the eastern sector of the Iberian Peninsula. Phytotaxa 172:61–70
Fu Y, Yang M, Horbach C, Kessler D, Diederichsen A, You FM, Wang H (2017) Patterns of SSR variation in bread wheat (Triticum aestivum L.) seeds under ex situ gene-bank storage and accelerated ageing. Genet Res Crop Evol 64:277–290. https://doi.org/10.1007/s10722-015-0349-9
Garnatje T, Garcia S, Canela MA (2007) Genome size variation from a phylogenetic perspective in the genus Cheirolophus Cass. (Asteraceae): biogeographic implications. Plant Syst Evol 264:117–134
Gianguzzi L, Papini F, Cusimano D (2015) Phytosociological survey vegetation map of Sicily (Mediterranean region). J Maps. https://doi.org/10.1080/17445647.2015.1094969
Gomez KA, Gomez AA (1984) Statistical procedures for agricultural research. John Wiley and sons Inc, New York
Greilhuber J, Temsch EM, Loureiro JCM (2007) Nuclear DNA content measurement. In: Doležel J, Greilhuber J, Suda J (eds) Flow cytometry with plant cells. Analysis of genes, chromosomes and genomes. Wiley-VCH Verlag, Weinheim, pp 67–101
Harkess A, Mercati F, Abbate L, McKain M, Pires JC, Sala T, Sunseri F, Falavigna A, Leebens-Mack J (2016) Retrotransposon proliferation coincident with the evolution of dioecy in asparagus. G3 Genes Genomes Genet 6(9):2679–2685
Hatch LC (2013) Cultivars of woody plants, 2.0th edn, vol 3. TCR Press, WI, p 114
Hewson K, Noormohammadi AH, Devlin JM, Mardani K, Ignjatovic J (2009) Rapid detection and non-subjective characterisation of infectious bronchitis virus isolates using high-resolution melt curve analysis and a mathematical model. Arch Virol 154(4):649–660. https://doi.org/10.1007/s00705-009-0357-1
Homma AKO (1992) The dynamics of extraction in Amazônia: a historical perspective. Adv Econ Bot 9:23–31
Homma AKO (1996) Utilization of forest products for amazonian development: potential and limitations. In: Lieberei R, Reisdorff C, Machado AD (eds) Interdisciplinary research on the conservation and sustainable use of the amazonian rain forest and its information requirements. Report on the workshop held in Brasilia, Brazil, November 20–22, 1995. Hamburg, Germany
Jiao Y, Jia H, Li X, Chai M, Jia H, Chen Z, Wang G, Chai C, Van de Weg E, Gao Z (2012) Development of simple sequence repeat (SSR) markers from a genome survey of Chinese bayberry (Myrica rubra). BMC Genom 13:201. https://doi.org/10.1186/1471-2164-13-201
Johnston JS, Bennett MD, Rayburn AL, Galbraith DW, Price HJ (1999) Reference standards for determination of DNA content of plant nuclei. Am J Bot 86:609–613
Jombart T, Ahmed I (2011) Adegenet 1.3-1: new tools for the analysis of genome-wide SNP data. Bioinformatics 27:3070–3071
Jordán MJ, Lax V, Martínez C, Aouissat M, Sotomayor JA (2011) Chemical intraspecific variability and chemotype determination of Rosmarinus officinalis L. in the region of Murcia. Acta Hort 925:109–114
Kamvar ZN, Tabima JF, Grünwald NJ (2014) Poppr: an R package for genetic analysis of populations with clonal, partially clonal, and/or sexual reproduction. Peer J 2:e281
Kellogg EA (1998) Relationships of cereal crops and other grasses. Proc Natl Acad Sci USA 95:2005–2010
Lamrani Alaoui M, Hassikou R (2018) Rapid risk assessment to harvesting of wild medicinal and aromatic plant species in Morocco for conservation and sustainable management purposes. Biodivers Conserv 27:2729–2745. https://doi.org/10.1007/s10531-018-1565-3
Le S, Josse J, Husson F (2008) FactoMineR: an R package for multivariate analysis. J Stat Softw 25:1–18. https://doi.org/10.18637/jss.v025.i01
Leitch IJ, Soltis DE, Soltis PS, Bennett MD (2005) Evolution of DNA amounts across land plants (Embryophytaz). Ann Bot 95:207–217
Lentini F, Venza F (2007) Wild food plants of popular use in Sicily. J Ethnobiol Ethnomed 3:15. https://doi.org/10.1186/1746-4269-3-15
Li G, Cervelli C, Ruffoni B, Shachter A, Dudai N (2016) Volatile diversity in wild populations of rosemary (Rosmarinus officinalis L.) from the Tyrrhenian Sea vicinity cultivated under homogeneous environmental conditions. Ind Crops Prod 84:381–390. https://doi.org/10.1016/j.indcrop.2016.02.029
Liu K, Muse SV (2005) Powermarker: an integrated analysis environment for genetic marker analysis. Bioinformatics 21:2128–2129
Mamoci E, Cavoski I, Simeone V, Mondelli D, Al-Bitar L, Caboni P (2011) Chemical composition and in vitro activity of plant extracts from Ferula communis and Dittrichia viscosa against postharvest fungi. Molecules 16:2609–2625. https://doi.org/10.3390/molecules16032609
Mateu-Andrés I, Aguilella A, Boisset F, Currás R, Guara M, Laguna E, Marzo A, Puche MF, Pedrola J (2013) Geographical patterns of genetic variation in rosemary (Rosmarinus officinalis) in the Mediterranean basin. Bot J Linn Soc 171:700–712. https://doi.org/10.1111/boj.12017
Médail F, Diadema K (2009) Glacial refugia influence plant diversity patterns in the Mediterranean Basin. J Biogeogr 36:1333–1345. https://doi.org/10.1111/j.1365-2699.2008.02051.x
Médail F, Quézel P (1999) Biodiversity hotspots in the Mediterranean Basin: setting global conservation priorities. Conserv Biol 6:1510–1513
Mercati F, Leone M, Lupini A, Sorgonà A, Bacchi M, Abenavoli MR, Sunseri F (2013a) Genetic diversity and population structure of a common bean (Phaseolus vulgaris L.) collection from Calabria (Italy). Genet Resour Crop Evol 3:839–852. https://doi.org/10.1007/s10722-012-9879-6
Mercati F, Riccardi P, Leebens-Mack J, Abenavoli MR, Falavigna A, Sunseri F (2013b) Single nucleotide polymorphism isolated from a novel EST dataset in garden asparagus (Asparagus officinalis L.). Plant Sci 203–204:115–123
Mercati F, Longo C, Poma D, Araniti F, Lupini A, Mammano MM, Fiore MC, Abenavoli MR, Sunseri F (2015) Genetic variation of an Italian long shelf-life tomato (Solanum lycopersicon L.) collection by using SSR and morphological fruit traits. Genet Resour Crop Evol 62:721–732. https://doi.org/10.1007/s10722-014-0191-5
Mercati F, Fontana I, Gristina AS, Martorana A, El Nagar M, De Michele R, Fici S, Carimi F (2019) Transcriptome analysis and codominant markers development in caper, a drought tolerant orphan crop with medicinal value. Sci Rep 9:2045–2322. https://doi.org/10.1038/s41598-019-46613-x
Migliore G, Saggio Scaffidi C (2007) La filiera delle piante officinali in Sicilia. In: Crescimanno M (ed) Le piante officinali in Sicilia Potenzialità di sviluppo della coltivazione con metodo biologico. Università degli Studi di Palermo Dip. ESAF, Palermo, pp 75–116 (in Italian)
Morales R (2010) Género Rosmarinus L. In: Morales R et al (eds) Flora iberica, Real Jardín Botánico, CSIC, Madrid, pp 327 331
Mulas M, Mulas G (2005) Cultivar selection from rosemary (Rosmarinus officinalis L.) spontaneous populations in the Mediterranean area. Acta Hort 676:127–133
Murray BG (2005) When does intraspecific C-value variation become taxonomically significant? Ann Bot 95:119–125
Napoli EM, Curcuruto G, Ruberto G (2010) Screening of the essential oil composition of wild Sicilian rosemary. Biochem Syst Ecol 4:659–670
Nei M (1978) Estimation of average heterozygosity and genetic distance from a small number of individuals. Genetics 89:583–590
Nunziata A, Cervelli C, De Benedetti L (2018) Genotype confidence percentage of SSR HRM profiles as a measure of genetic similarity in Rosmarinus officinalis. Plant Gene 14:64–68
Nunziata A, De Benedetti L, Marchioni I, Cervelli C (2019) High resolution melting profiles of 364 genotypes of Salvia rosmarinus in 16 microsatellite loci. Ecol Evol 9:3728–3739
Peakall R, Smouse PE (2006) GenAlEx6: genetic analysis in Excel. Population genetic software for teaching and research. Mol Ecol Notes 6:288–295
Pellicer J, Estiarte M, Garcia S, Garnatje T, Peñuelas J, Sardans J, Vallès J (2010) Genome size unaffected by moderate changes in climate and phosphorus availability in Mediterranean plants. Afr J Biotech 9(37):6070–6077
Petrov DA, Sangster TA, Johnston JS, Hartl DL, Shaw KL (2000) Evidence for DNA loss as a determinant of genome size. Science 287:1060–1062
Pignatti S (1982) Flora d'Italia, vol II. Edagricole:500, Bologna
Pintore G, Usai M, Bradesi P, Juliano C, Boatto G, Tomi F, Chessa M, Cerri R, Casanova J (2002) Chemical composition and antimicrobial activity of Rosmarinus officinalis L. oils from Sardinia and Corsica. Flavour Fragr J 17:15–19
Pritchard JK, Wen W (2003) Documentation for STRUCTURE software version 2. https://web.stanford.edu/group/pritchardlab/software/readme/readme.html. Accessed 20 Dec 2019
Rosselló JA, Cosín R, Boscaiu M, Vicente O, Martínez I, Soriano P (2006) Intragenomic diversity and phylogenetic systematics of wild rosemaries (Rosmarinus officinalis L. s.l., Lamiaceae) assessed by nuclear ribosomal DNA sequences (ITS). Plant Syst Evol 262:1–12
Rosúa JL (1981) El complejo Rosmarinus eriocalyx-tomentosus en la península ibérica. Anales Jard Bot Madrid 2:587–595
Salido S, Altarejos J, Nogueras M, Sanchez A, Luque P (2003) Chemical composition and seasonal variations of rosemary oil from Southern Spain. J Essent Oil Res 15:10–14
Sánchez-Camargo AdP, Herrero M (2017) Rosemary (Rosmarinus officinalis) as a functional ingredient: recent scientific evidence. Curr Opin Food Sci 14:13–19
Schippmann U, Leaman DJ, Cunningham AB (2002) Impact of cultivation and gathering of Medicinal Plants on biodiversity: global trends and issues. In: FAO, 2002, “Biodiversity and the ecosystem approach in Agriculture, Forestry and Fisheries”, Inter-Departmental Working Group on Biological Diversity for Food and Agriculture, Rome, p 21
Segarra-Moragues JG, Gleiser G (2009) Isolation and characterisation of di and tri nucleotide microsatellite loci in Rosmarinus officinalis (Lamiaceae), using enriched genomic libraries. Conserv Genet 3:571–575
Sgorbini B, Bicchi C, Cagliero C, Cordero C, Liberto E, Rubiolo P (2015) Herbs and spices: characterization and quantitation of biologically-active markers for routine quality control by multiple head space solid-phase microextraction combined with separative or non-separative analysis. J Chromatogr A 1376:9–17
UPOV (2000) Working paper on test guidelines for Rosemary (Rosmarinus officinalis L.). Technical working party for vegetables, thirty-fourth session, Angers, France, September 11–15, 2000. https://www.upov.int/edocs/mdocs/upov/en/twv/34/twv_34_14.pdf Accessed 10 Sep 2019
Varela F, Navarrete P, al R, Fanlo M, Melero R, Sotomayor JA, Jordán MJ, Cabot P, Sánchez de Ron D, Calvo R, Cases A (2009) Variability in the chemical composition of wild Rosmarinus officinalis L. Acta Hort 826:167–174
Zaouali Y, Boussaid M (2008) Isozyme markers and volatiles in Tunisian Rosmarinus officinalis L. (Lamiaceae): a comparative analysis of population structure. Biochem Syst Ecol 36:11–21
Zaouali Y, Chograni H, Trimech R, Boussaid M (2012) Genetic diversity and population structure among Rosmarinus officinalis L. (Lamiaceae) varieties: var. typicus Batt. and var. troglodytorum Maire. based on multiple traits. Ind Crops Prod 38:166–176
Zaouali Y, Messaoud C, Ben Salah A, Boussaïd M (2005) Oil composition variability among populations in relationship with their ecological areas in Tunisian Rosmarinus officinalis L. Flavour Fragr J 20:512–520
Acknowledgements
The collection field was carried out within the Project “Biodiversity preservation—Public Conservation Centers—Safeguard and exploitation of Sicilian herbaceous crops populations and varieties”. PSR Sicilia 2007–2013: Misura 214/2, Azione A.
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Carrubba, A., Abbate, L., Sarno, M. et al. Characterization of Sicilian rosemary (Rosmarinus officinalis L.) germplasm through a multidisciplinary approach. Planta 251, 37 (2020). https://doi.org/10.1007/s00425-019-03327-8
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DOI: https://doi.org/10.1007/s00425-019-03327-8